Evaluation of effect of growth factor on tendon strength with plasma irradiation

Subject Areas :

alireza omranifard ¹ , Alireza Jahandideh ² , Ahmad Asghari ³ , Saeed Hesaraki ⁴

1 - Department of Clinical Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2 - Department of Clinical Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - Department of Clinical Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
4 - Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Received: 2024-09-08 Accepted : 2024-11-13 Published : 2024-12-20

Keywords: Tendon healing, Fibroblast Growth Factor, Cold Atmospheric Plasma, tendon tensile strength, histopathology,

Abstract :

Nowadays, treatment of tendons injury and restoring their function in humans and animals is one of the medical challenges. This study investigating effect of simultaneous treatment of fibroblast growth factor-2 (bFGF) and cold atmospheric plasma (CAP) on injured Achilles tendon in rabbits in order to evaluate effect on tensile tolerance and histological quality of repaired tendon tissue. For this purpose. In the first experiment group 0.2 ml distilled water injected proximal and distal of injury site for control. The second group bFGF was performed according to the first experiment, with the difference that (10 µg) bFGF in 0.2 ml of distilled water was injected. In the third group CAP Achillis tendon expose to CAP from the skin surface for 30 seconds for 21 consecutive days. fourth group (CAP–bFGF) were treated both CAP and bFGF. According to the obtained results after 120 days simultaneous treatment (CAP–bFGF) group showed significant histological improvement compared to the control group ( P< 0.001 ) and independent treatment groups (P < 0.05). also, it showed higher endurance in tensile test with average bearing force of (125.04 N) compared to other test groups ( P< 0.001 ) Finally, the analysis of the obtained results showed that CAP and bFGF independently improve the healing and strength of tendon, but no significant difference was seen between their effectiveness. whereas simultaneous use of fibroblast growth factor and atmospheric cold plasma radiation leads to enhancement of the therapeutic effect and tendon tensile strength as compared to independent treatments.

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