The effects of oyster mushroom and bacto-gene probiotic on blood parameters in broiler chickens
Subject Areas : Shelf Life of Food Products and Agricultural ProductionsAbolfazl Asadi Dizaji 1 , Hamed Shirandaz Asli 2 , Tovhid Vahdatpour 3
1 - Department of Animal Science, Shabestar Branch, Islamic Azad University, Shabestar, Iran
2 - Department of Animal Science, Shabestar Branch, Islamic Azad University, Shabestar, Iran
3 - Department of Animal Science, Shabestar Branch, Islamic Azad University, Shabestar, Iran
Keywords: Blood parameters, Broiler, Oyster mushroom, Probiotic bacto-gene,
Abstract :
Medicinal plants help to absorb nutrients and stimulate appetite in poultry. The aim of this study was to determine the effect of oyster mushroom and bacto-gen probiotics on blood parameters in broiler chickens. A total of 360-day-old commercial broiler chicks (Ross 308) in the factorial design of 2*5 with two levels of probiotics (zero and 0.04%) and five levels of oyster mushrooms (zero, 1, 2, 3, and 4 percent) in a randomized complete design. were distributed randomly into ten groups and three repetitions with 12 chickens in per pen. The chickens had the same environmental and management conditions as the experimental groups and fed special diets for each treatment for 42 days. The results showed that mushroom level had no significant effect on LDL, HDL, TG, RBC, HCT, HB, lymphocyte, monocyte, basophil and eosinophil, but significantly decreased cholesterol, heterophil, heterophil to lymphocyte ratio and increased significantly WBC (P>0.05). Probiotic inclusion had no significant effect on HDL, RBC, HCT, HB, monocyte, eosinophil and basophil, but significantly decreased TG, LDL, heterophil, heterophil to lymphocyte ratio, cholesterol and increased significantly WBC and lymphocyte count (P>0.05). Therefore, it was concluded that the addition of edible mushrooms and probiotic bacto-genes to the diet of broiler chickens increases beneficial intestinal flora, stimulates growth, produces healthy products and uses less antibiotics in broiler diet.
1- Kanchana G and Jeyanthi G. P. Influence of vitamin E and selenium supplementation on the growth performance and antibody responses of layer chicks. International Journal of Pharmacy and Biological Sciences, 2010; 2: 1-9.
2- Abd El -Latif SA, El -Yamany AT, Eman AF. Evaluation of using different levels and sources of medicinal herbs in growing Japanese quail diets. Egyptian Journal of Nutrition and Feeds, 2004; 7: 69 -81.
3- Zhang D, Hu H, Rao Q, Zhao Z. Synergistic effects and physiological responses of selected bacterial isolates from animal feed to four natural antimicrobials and two antibiotics. Food borne Pathogens and Disease, 2011; 8:1055–1062.
4- Endo Mahata M, Syahputra Ritonga I, Hidayat K, Batubara L, Habiyah U, Rizal.Y. The supplementation effect of oyster mushroom (Pleurotus ostreatus) in broiler's diet on their performance and cholesterol.19th Europ. Symp. Poultry. Nutrition. Potsdam, Germany, 2013; 1-5.
5- Mahfuz S, He T, Jiayu Ma, Hansuo Liu, Long S, Shang Q, Lianhua Zhang, Jingdong Yin, Piao X. Mushroom (Flammulina velutipes) stem residue on growth performance, meat quality, antioxidant status and lipid metabolism of broilers Italian Journal of Animal Science, 2020; 19: 803-812.
6- Navarro-Simarro P, omez-G G, Omez L, Ahrazem O, Rubio-Moraga A. Food and human health applications of edible mushroom by-products. N. Biotechnology. 2024; 81:43–56.
7- Kim Y H, Jung E G, Han K I, Patnaik B B, Kwon H J, Lee H S, Kim W J, Han M D. Immunomodulatory effects of extracellular and beta-glucan isolated from the king oyster mushroom Pleurotus eryngii (Agaricomycetes) and its sulfated form on signaling molecules involved in innate immunity. International Journal of Medicinal Mushrooms, 2017; 19:521–533.
8- Asadi Dizaji A, Aghdam Shahryar H, Shaddel Tili A, Maheri Sis N, Ghiasi Ghalehkandi J. Effect of level of white button mushroom (Agaricus. bisporus) on blood biochemical characteristics of Japanese quails (Coturnix japonica). Journal on New Biological Reports, 2014; 3(3): 175–181.
9-Toghyani M, Tohidi M, Gheisari A, Tabeidian A, Toghyani M. Evaluation of oyster mushroom (Pleurotus Ostreatus) as a biological growth promoter on performance, humoral immunity, and blood characteristics of broiler chicks. The Journal of Poultry Science, 2014; 49:183-190.
10- Willis WL, Isikhuemhen OS, Allen JW, Byers A, King K, Thomas C. Utilizing fungus myceliated grain for molt induction and performance in commercial laying hens. Poultry Science, 2009; 88: 2026-2032.
11- Boysinova N, Ibragimov F, Yunusov K, Achilov O, Rasulov U. The effectiveness of using probiotics, their effect on growth and chemical composition of broiler chicken meat. BIO Web of Conferences 95, 01013 (2024) CIBTA-III-2024.
12- Muhamediyeva DT, Safarova LU, Tukhtamurodov N. Early diagnostics of animal diseases on the basis of modern information technologies, AIP Conference Proceedings, 2817, 020038 (2023).
13- knowledge group Takgen. Takgene was established in 2004 as a science-based company in order to produce different kind of probiotic products. This company is the sole company in the country that is dedicated to produce various types of functional microorganisms for food industry, 2004. www.takgene.com.
14- National Research Council. Nutrient requirements of dairy cattle, Seventh revised edition, National academy press, Washington. D.C. 1994.
15- Asadi Dizaji A, Aghdam Shahryar H, Shaddel Tili A, Maheri Sis N, Ghiasi Ghalehkandi J. Effect of feeding common (Agaricus bisporus) and oyster (Pleurotus ostreatus) mushrooms on performance, Intestinal microbiology and morphology of female Japanese quails (Coturnix coturnix japonica). Veterinarija ir Zootechnika, 2023; 81(1): 33-38.
16- Bormon CC, Akib G, Rifat A, Hossain M, Uddin N, Hossain FMA, Azzam MM, Farouk MH, Das R and Mahfuz SU. Effects of oyster mushroom (Pleurotus ostreatus) stem residue supplementation on growth performance, meat quality and health status of broilers. Poultry Science, 2024; 103:104054.
17- SAS Institute. SAS Users Guide Statistics, SAS Institute Inc. Cary, NC. 2001.
18- Khatun K, Mahtab H, Khanam P A, Sayeed MA and Khan KA. Oyster mushroom reduced blood glucose and cholesterol in diabetic subjects. Mymensingh Medical Journal, 2007; 16: 94-99.
19- Fukushima M, Ohashi T, Fujiwara Y, Sonoyama K, Nakano M. Cholesterol lowering effects of maitake (Flammulinavelu tipes) fiber in rats. Experimental biology and medicine (Maywood), 2001; 226: 758-765.
20- Daneshmand A, Sadeghi GH, Karimi A. The effects of a combination of garlic, oyster mushroom and propolis extract in comparison to antibiotic on growth performance, some blood parameters and nutrients digestibility of male broilers. Brazilian Journal of Poultry Science, 2012; 14(2): 184-191.
21- Londok J J M, Rompis J E G. Hematological parameters in broiler chicken consumed lauric acid and feed fiber. 9th International. Sem Atlantis Press, 2022; 144–147 Trop. Animal Product. (ISTAP 2021).
22- Willis WL, Isikhuemhen OS, Hurley S, Ohimain EI. Effect of phase feeding of fungus myceliated grain on oocyst excretion and performance of broiler chicken. International Journal of Poultry Science, 2011; 814: 101-103.
23- Basko D. The many health benefits of mushrooms (and how to add them to your pet's diet). 2014. (http://www.¬drbasko.-com/¬site/¬pets- mushrooms-health-benefits).
24- Kamruzzaman SM, Kabir SML, Rahman M M, Islami MW, Rezai MA. Effect of probiotics and antibiotics supplementation on body weight and certain haemato-biochemical parameters in broilers. Bangladesh Journal of Veterinary Medicine, 2005; 3¬ (2): 100-104.
25- Mahfuz S, He T, Jiayu Ma, Hansuo Liu, Long S, Shang Q, Lianhua Zhang, Jingdong Yin, Piao X. Mushroom (Flammulina velutipes) stem residue on growth performance, meat quality, antioxidant status and lipid metabolism of broilers Italian Journal of Animal Science, 2020; 19, 803-812.
26- Ashayerizaded A, Dabiri N, Mirzadeh KH and Ashayerzadeh A. The comparison between antibiotics, probiotics and prebiotics on growth response and some blood parameters in 42 days old broiler. Proceed. 4th Iranian Congress Animal Science Tehran, Iran. 2010; 786-789.
27- Ooi LG, Liong MT. Cholesterol-lowering effects of probiotics and prebiotics: A review of in vivo and in vitro findings. International Journal of Molecular Sciences, 2010; 11: 2499-2522.
28- Thiam M, Wang Q, Barreto Sánchez AL, Zhang J, Ding J, Wang H, Zhang Q, Zhang N, Wang J, Li Q, Wen J. Heterophil/lymphocyte ratio level modulates Salmonella resistance, cecal microbiota composition and functional capacity in infected chicken. Frontiers in Immunology. 2022; 13:816689.
29-Javid MA, Masood S, Zaneb H, Rehman HU, Nazir MM, Waqas MY, Sufyan AB. Effect of dietary growth promoter supplementations on the serum biochemistry and morphometric characteristics of tibia bone in broiler chicken. Brazilian Journal of Poultry Science. 2022, 24, eRBCA-2021.
30- Al-Saad S, Abbod M, Abo Yones A. Effects of some growth promoters on blood hematology and serum composition of broiler chickens. International Journal on Agriculture Research, 2014; 9: 265-270.
31- Aliakbarpour HR, Chamani M, Rahimi G, Sadeghi AA, Qujeq D. Intermittent feeding program and addition of Bacillus subtilis based probiotics to the diet of growing broiler chickens: Influence on growth, hepatic enzymes and serum lipid metabolites profile. Archiv Tierzucht, 2013; 56 (40): 410-422.
32- Zareshahneh AR, Abdelahi MR, Kamyab AR, Nikkhah A. Effect of different levels of bacterial probiotic on broilers performance and some of the blood factors. Journal Agriculture and Natural Resources Sciences, 2007; 14(4): 162- 170.
33- Karoglu M, Durdag H. The influence of dietary probiotic (Sacchromyces cerviciae) supplementation and different slaughter age on the performance, slaughter and carcass properties of broiler. International Journal of Poultry Science, 2005; 4: 309-316.
