Effects of addition of glutamine to semen extenders on microscopic factors of buffalo bulls sperm after thawing

Subject Areas : Journal of Comparative Pathobiology

رحیم بهشتی ¹ (ندارد)
مجید یوسفی اصل ² (ندارد)
جمشید قیاسی ³ (ندارد)

Received: 2012-05-09 Accepted : 2012-07-31 Published : 2012-11-21

Keywords: Sperm, buffalo, glutamine, semen freezing,

Abstract :

The aim of this study was to evaluate the effects of the addition of different levels of antioxidant(glutamine) on buffalo spermatozoa cryopreserved in an egg yolk based extender. Ejaculates werecollected From 5 matures buffalo bulls (four ejaculate per buffalo), were evaluated and diluted at37°C in one of the following experiments: Tris-egg yolk extender (control), or the same extendersupplemented with 10, 25, 50, 75 or 100 mM glutamine. The semen was loaded into 0.5 mlstraws, cooled and frozen in a manual freezer and subsequently stored in liquid nitrogen. Prior toevaluation, frozen straws were thawed in a water bath (37◦C for 20 s). Freezing extenderssupplemented with 25 mM glutamine led to higher sperm motility values, compared to the controland other groups. The addition of antioxidants did not affect acrosomal integrity compared to thecontrol. The percentage of sperm viability in extender supplemented with 25 mM glutamine washigher than control group (78.03 ±0.37 & 53.13 ±0.40, respectively). The sperm viability inextender supplemented with 100 mM glutamine was lower than other glutamine supplementedgroups. In conclusion, addition of glutamine to buffalo semen extender laded to higher frozensperm quality in buffalo.