Effect Fenugreek seeds hydroethanolic extract on a full-thickness wound healing in Streptozotocin-induced diabetic mice
Subject Areas : Journal of Comparative Pathobiology
Farahpour, M.R., Aghaei, M. .
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Keywords: Fenugreek seed, Hydroethanolic extract, Excisional wound healing, Diabetic mice,
Abstract :
Disturbance in wound healing process is considered to be one of the most serious complications of diabetes, because it significantly increases the susceptibility of patient wounds to be infected. Fenugreek is an annual plant which its leaves and seeds used extensively in foods and has advantages to human health. Due to prevalence rate and side effects of diabetes, Fenugreek seed extract is one of medicinal herb have been used more frequently. The present study was performed to investigate the effects of using fenugreek seed hydroethanolic extract on wound healing process in the 54 mice model of diabetes type 2. One single full-thickness excisional wound was created with 6-mm punch biopsy on back of each mice. All animal, accidentally, divided in three groups: Control group, 10% and 5% fenugreek seed hydroethanolic extract treatment groups. In terms of microscopic, the rate of tissue edema, neo-vascularization, fibroblast infiltration, collagen deposition and re- epithelialization were evaluated. Results revealed that the edema score significantly decreased (P<0.05) in both treatment groups compared to the control group, whereas the neo-vascularization, fibroblast infiltration, collagen deposition and re- epithelialization significantly increased (P<0.05) in both treatment groups compared to the control group. These results showed that topical administration of fenugreek seed hydroethanolic extract promote skin full-thickness excision wound healing process in diabetic animal model.
