Molecular isolation of Salmonella from faeces of the street cat by PCR method

Subject Areas : Journal of Comparative Pathobiology

Sadeghi Vafa, F. , Mashhady Rafie, S. , Jamshidian, M. . ¹ (.)

Received: 2017-02-12 Accepted : 2017-02-12 Published : 2017-03-21

Keywords: Salmonella enteritidis, Serotyping, Cat, Tehran,

Abstract :

This study was performed for detecting enteric Salmonella in stray cats in Tehran. Stool samples obtained from 100 stray cats were referred to small animal clinics by SPCA members from different part of Tehran. Routine culture, serotyping test and molecular methods (PCR and M-PCR) were done for evaluating of genius and serovars of bacteria. Samples were placed in 37 ˚C for 24 hours in selective media such as Rambach medium. After the colonies appeared, they cultured in differential media such as TSI. Results recorded after 24 hours later. Only one sample showed Salmonella Spp. in culture method from a 2 month old kitten with hemorrhagic enteritis and severe dehydration. This sample and other suspected cases were tested by PCR and M-PCR subsequently. We found another case of Salmonella enteritidis from a female one-year-old cat without any clinical signs. Serotyping tests showed both cases belong to groupD1. Although Salmonella enteritidis is an important factor in human gastroenteritis and recorded from poultry farms and products, it is supposed to detecting this kind of Salmonella are created after exposing to related resources.