Effects of different levels of salinity on number and size of chloride cells in gill of Benny fingerling (Barbussharpeyi)

Subject Areas : Journal of Comparative Pathobiology

Koohkan, O. . ¹ (.)

Received: 2017-02-12 Accepted : 2017-02-12 Published : 2017-03-21

Keywords: Barbussharpeyi, Gill, Choloride cell, Salinity, Osmoregulation,

Abstract :

This study was accomplished to evaluate osmotic regulation ability of Benny fish by mitochondria-rich cells and study of the effect of salinity in different levels on their gill. For this purpose, 120 fish transferred into four aquariums containing 4, 8, 10 and 12 g/l salinity, after a week adaptation. Sampling was carried out after 96hrs and tissues prepared and fixed in 10% formalin for 48hrs. After tissue processing, 5µm sections were prepared and stained with haematoxylin and eosin and studied by light microscope. The observations showed most presence of chloride cells in basement of filament and lower in lamellae of Barbussharpeyi. Chloride cells and mucosal cells increased in size and number with salt concentrations increasing and showed significant different in some treatments (P<0.05). Mucosal cell number in12 g/l concentration showed significant increasing compared to both control group and 4 g/l concentration, but no significant difference with other treatments. In chloride cells, only 12 g/l treatment showed a significant difference (p<0/05) compared to control group in number and size.