Effect of lethal exposure of lead acetate on histopathology of gills of probiotic- treated zebra fish (Danio rerio)

Subject Areas : Journal of Comparative Pathobiology

, F. Tehrani ¹ (.Department of Biology, Roudehen Branch,Islamic Azad University, Roudehen, Iran)
N. Hadizadeh Shirazi, ² (Department of Biology, Roudehen Branch,Islamic Azad University, Roudehen, Iran)
, R. Kazempoor ³ (Department of Biology, Roudehen Branch,Islamic Azad University, Roudehen, Iran)

Received: 2020-11-07 Accepted : 2020-11-07 Published : 2020-05-21

Keywords: Zebrafish, Lead, Lethal dose, Probiotic, Lactobacillus Fermentum,

Abstract :

For this purpose, 360 Zebra fish were divided into two groups and fed for 60 days with commercial food (control group) or enriched diet with Lactobacillus fermentum as probiotic (test group). After this time, both groups were in the vicinity of 0.3 mg / l of lead and their survivability, tissue metal uptake and their histological damages were studied within 48 hours. Based on the results, the fish in the test group were able to tolerate the lethal dose of lead for more than 24 hours compared to the control group. After 48 hours of poisoning, the amount of metal uptake in both liver and gill tissue was significantly lower for the experimental group than in the control group. Moreover, tissue damage such as vein dilatation, lamella fusion and telangiectasia were significantly lower in the experimental group than the control group. Epithelial cell hyperplasia was not observed in the probiotic- treated group. It could be concluded that probiotic enriched diet can significantly reduce the toxic effects of lethal doses of lead in fish.

References:

1. Kurniawan,T.A . Comparison of low-cost adsorbent for treating wastewater ladenwith heavy metals. Science of the Total Environment.2006, 366: 409-426.


2. Al-yaqout,A. F. Assessment and analysis of industrial liquid waste and sludge disposal at unlined landfill sites in arid climate. Waste management. 2003, 23. pp: 817-824.


3. Sanders T, Liu Y, Buchner V, Tchounwou PB. (2009). Neurotoxic eff ects and biomarkers of lead exposure: A Review. Res Environ Health 24: 15–45.


4. Flora G, Gupta D, Tiwari A. Toxicity of lead:A review with recent updates. Interdiscip. Toxicol. 2012;5(2):47–58.


5. Al-Balawi, H.F. Al-Akel, A.S. Al-Misned, F. Suliman, A.M. Effects of sub-lethal exposure of lead acetate on histopathology of gills, liver, kidney and muscle and its accumulation in these organs of Clarias gariepinus. Brazilian Archives of Biological Technology. 2013, 56(2): 27-31.


6. Savard P, Lamarche B, Paradis M, Thiboutot H, Laurin E, Roy D. Impact of Bifidobacterium animalis subsp. lactis BB- and, Lactobacillus acidophilus LA-5- containing yoghurt, on fecal bacterial counts of healthy adults. International Journal of Food Mcrobiology2011. 149(1):50-57.


7. Stanton C, Gardiner G, Meehan H, et al. Market potential for probiotics. American Journal of Clinical Nutrition. 2001,73(2): 476S- 483S.


8. Guo H, Luo S, Chen L, Xiao X, Xi Q, Wei W, et al. Bioremediation of heavy metals by growing hyperaccumulaor endophytic bacterium Bacillus sp. L14. Bioresource Technology .2010, 101(22):8599-605


9. Saaveda JM. Clinical applications of probiotic agents. American Journal of Clinical Nutrition. 2001, 73(6): 1147S-51S


10. Haffter GM, Brand M, Mullins MC, Hammerschmidt M, Kane DA, Odenthal J, van Eeden FJM, Jiang Y, Heisensberg C, Kelsh RN . The identification of genes with unique and essential functions in the development of the zebrafish, Danio rerio. Development.1996, 123:1-36.


11. Driever W, Solnica-Krezel L, Schier AF, Neuhauss SCF, Malicki J, Stemple DL, Stainier DYR, Zwartkruis F, Adbelilah S, Rangini Z . A genetic screen for mutations affecting embryogenesis in zebrafish. Development 1996, 123:37-46.


12. Bahary N, Zon LI. Use of the zebrafish (Danio rerio) to define hematopoiesis. Stem Cells.1998, 16:89-98.


13. Chen JN, Haffter P, Odenthal J, Vogelsang E, Brand M, van Eeden FJ, Furutani-Seiki M, Granato M, Hammerschmidt M, Heisenberg CP . Mutations affecting the cardiovascular system and other internal organs in zebrafish. Development. 1996,123:293-302.


14. Dressler G. Kidney development branches out. Developmental Genetics.1999, 24:163-189.


15. Mohammadian, T., Alishahi, M., Tabandeh, M. R., Ghorbanpoor, M., Gharibi, D., Tollabi, M. and Rohanizade, S.Probiotic effects of Lactobacillus plantarum and L. delbrueckii ssp. bulguricus on some immune-related parameters in Barbus grypus. Aquaculture International.2016, 24(1): 225–242


16. Rayes, A. A. H. Field studies on the removal of lead, cadmium and copper by the use of probiotic lactic acid bacteria from the water for culturing marine tilapia T. spilurus. New York Science.2012, 5(11): 74-82

 

17. Giri SS, Yun S, Jun JW, Kim HJ, Kim SG, Kang JW. Therapeutic Effect of Intestinal Autochthonous Lactobacillus reuteri P16 Against Waterborne Lead Toxicity in Cyprinus carpio. Front. Immonology.2018, 9:1824.


18. Abu-Braka1 A , Saad Zaki M, Hassan Abbas H , Ahmed Ismail N, Khalil R, Tanekhy M. Filed studies on some probiotics to minimize hazard effects of prevailing heavy metals contamination for improving immunity and growth performance of Oreochromis niloticus Electronic Physician.2017, 9(4):4134-4144.


19. Musawi A, Johari W, Ikhsan N.The Growth Potential and Bioaccumulation Ability of Probiotics under the Exposure of Different Heavy Metals. Pertanika Journal of Tropical Agriculture Sciences. 2019, 42 (1): 305 - 314


20. Najafi Enferadi, M.H. Mohammadizadeh, F. Soltani, M. Bahri, A.H. Sheikhzadeh, N. Effects of Lactobacillus plantarum and Mannan Oligosaccharide on growth performance and some digestive factors in Oncorhynchus mykiss. Animal physiology and development. 2020; 13(48): 69-83


21. Hosseini, A. Oraji, H. Yegane, S. Shahabi, H. The effect of probiotic Pediococcus acidilactici on growth performance, blood and some serum parameters in Caspian salmon (Salmo trutta caspius). Iranian Scientific Fisheries Journal, 2014 2:35-45.


22. Panahi Sahebi, H., Esmaeili Fereidouni, A., Imanpour, M., Taheri Mirghaed, A., Barari, A., Kavianpour, M. Effects of Dietary Inclusion of Prebiotic Immunowall and Probiotic Primalac on Growth Indices, Survival, Body Composition, and Blood Biochemical Parameters in the Caspian Sea Carp, Cyprinus carpio, Fingerlings. Journal of Veterinary Research, 2019; 74(1): 45-53.


23. Abdullah al mamun, M, Nasreni SH, Bari S. 2018. Role of probiotics in aquaculture: importance and future guidelines. Journal of Bangladesh Academic Sciences 42 (1):105-109


24. Rahimibashar M.R., Alipoor V., Danesh M., Alinia M.R. Survival of biometrical characteristics, diet, gonad and liver index of (Sander lucioperca) in the lake of Arass Dam. Pajouhesh and Sazandegi. 2008, 79: 58-65


25. Shakoori, M. Abdali, S. Effect of Lead on Some Biochemical Indices of Farmed Silver Carp (Hypophthalmichthys molitrix).2016.Journal of Marine Science and Technology Research. 1:1-12


26. Mohamadzadeh, P., Gamili, S. Effect of PbNO3 on Gill and Liver tissues in Rutilus rutilus. Applied Biology, 2014; 27(1): 79-96.


27. Mahboob Sh, Kausar Sh, Jabeen F, Sultana, S. Effect of Heavy Metals on Liver, Kidney, Gills and Muscles of Cyprinus carpio and Wallago attu inhabited in the Indus. Brazilian Archives of Biology and Technology.2016, 59, e16150275


28. Zhai Q, Yang L, Zhao J, Zhang H, Tian F and Chen W .Protective Effects of Dietary Supplements Containing Probiotics, Micronutrients, and Plant Extracts Against Lead Toxicity in Mice. Front. Microbiology. 2018, 9:2134.


29. Abu-Braka AZ, Zaki MS, Abbas HH, et al. Filed studies on some probiotics to minimize hazard effects of prevailing heavy metals contamination for improving immunity and growth performance of Oreochromis niloticus. Electron Physician. 2017;9(4):4138-4144.


30. Mahious, A.S., Ollevier, F. (2005). Probiotics and prebiotics in aquaculture: review. 1st regional workshop on techniquesfor enrichment of live food for use inlarviculture. AAARC, pp. 17-26 (Urmia, Iran).


31. Denis F, Archambault D. Molecular cloning and characterization of beluga whale (Delphinapterus leucas) interleukin-1b and tumor necrosis factor-a. Canadian Journal of Veternity Reseaarch.2001, 65:233–40


32. Clark R.B.Marine Pollution. Oxford University Press, 248 P. Dobaradaran S., Naddafi K., Nazmara S.H., Ghaedi H. 2010. Heavy metals (Cd, Cu, Ni and Pb) content in two fish species of Persian Gulf in Bushehr Port, Iran. African Journal of Biotechnology.2001, 9(37): 6191‐6193


33. Irianto, A., Austin, B., 2002, Probiotics in aquaculture. Journal of Fish Diseases. 25, pp: 633 – 642.


34. Ferguson R., Merrifield D.L., Harper G.M., Rawling M.D., Mustafa S., Picchietti S., Balcazar J.L. and Davies S.J. 2010. The effect of Pediococcus acidilactici on the gut microbiota and immune status of on-growing red tilapia (Oreochromis niloticus). Journal of Applied Microbiology, 109(3): 851–862.


35. Abalaka S E .Heavy metals bioaccumulation and histopathological changes in Auchenoglanis occidentalis fish from Tiga dam, Nigeria, Journal of Environmental Health Science & Engineering.2015, 13:67-71.


36. Jahanbakhshi A., Hedayati A. Gill histopathological changes in Great sturgeon after exposure to crude and water soluble fraction of diesel oil. Comparative Clinical Pathology.2012, 22 (6): 1083-1086


37. Rosety-Rodríguez M., Ordoñez F.J., Rosety M., Rosety J.M., Ribelles A., Carrasco C. Morpho-histochemical changes in the gills of turbot, Scophthalmus maximus L., induced by sodium dodecyl ulfate. Ecotoxicology and Environmental Safety.2002, 51: 223-228


38. Khaleghi, R. Hedayati, A.K. Kashiri, H. Paknejad, H. Hosseinifar, H. Effect of Pediococcus acidilactici probiotic and Agaricus bisporus prebiotic supplements on the expression of immune-related genes in common carp (Cyprinus carpio) exposed to silver nanoparticles,2019. Aquatic Physiology and Biotechnology, 6(4): 155-174.


39. Sayes C, Leyton Y, Riquelme C.Probiotic Bacteria as Healthy Alternative for Fish Aquaculture, Antibiotic Use in Animals, Sara Savic, IntechOpen,2017 DOI: 10.5772/intechopen.71206.