Decellularization of ostrich plantar ligament

Subject Areas : Journal of Comparative Pathobiology

روزبه Moridpour ¹ (Department of Clinical Science, Science and Research Branch, Islamic Azad University, Tehran, Iran)
حمیدرضا Fattahian ² (Department of Clinical Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.)
پژمان Mortazavi ³ (Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran.)

Received: 2022-10-01 Accepted : 2023-02-11 Published : 2023-02-11

Keywords: Decellularization, Ligamentum plantar, Ostrich,

Abstract :

The use of tissue is very common to return organ function nowadays. Tissue engineering and novel preparation techniques in therapy are a main role. In present research use of chemical and physical methods was tried to prepare tissue from connective tissue of plantar structure of ostrich. Five pair of ostrich is feet separated of tarsometatarsal joint were sent to laboratory. Microscopic study and biomechanical evaluation were performed. De-celluraization of the tissue was done with sodium dodecyl sulfate and vacuum. Finally, light and electron microscope studies were carried out. Biomechanical evaluation was done as well. Ligamentum plantar composed of fibrocartilage tissue that decellularization process last as percent of cells and their nucleus content. There was not significant difference between prepared tissue and original tissue in tension and compression parameters. SDS with vacuum decellularized till as percent with connective tissue unchanged with minimal antigenicity feature as xenograft. The tissue could be used after sterilization in patient in future.

References:

1. Banigo AT, Iwuji SC, Iheaturu NC. Application of biomaterials in tissue engineering: a review. Journal of Chemical and Pharmaceutical Research. 2019;11(4):1-16.


2. Kim TH, Yan J-J, Jang JY, Lee G-M, Lee S-K, Kim BS, et al. Tissue-engineered vascular microphysiological platform to study immune modulation of xenograft rejection. Science Advances. 2021;7(22):eabg2237.


3. Fabbriciani C, Lucania L, Milano G, Panni AS, Evangelisti M. Meniscal allografts: cryopreservation vs deep-frozen technique An experimental study in goats. Knee Surgery, Sports Traumatology, Arthroscopy. 1997;5(2):124-34.


4. Keane TJ, Swinehart IT, Badylak SF. Methods of tissue decellularization used for preparation of biologic scaffolds and in vivo relevance. Methods. 2015;84:25-34.


5. Hopf A, Al-Bayati L, Schaefer DJ, Kalbermatten DF, Guzman R, Madduri S. Optimized Decellularization Protocol for Large Peripheral Nerve Segments: Towards Personalized Nerve Bioengineering. Bioengineering. 2022;9(9):412.


6. Narita Y, Kagami H, Matsunuma H, Murase Y, Ueda M, Ueda Y. Decellularized ureter for tissue-engineered small-caliber vascular graft. Journal of Artificial Organs. 2008;11(2):91-9.


7. McInnes AD, Moser MA, Chen X. Preparation and Use of Decellularized Extracellular Matrix for Tissue Engineering. Journal of Functional Biomaterials. 2022;13(4):240.


8. Moridpour R, Fattahian H, Mortazavi P. Using Cell-free Matrix Derived from Ostrich Plantar Ligament for Repair of Articular Cartilage Defect of Rabbit. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2017;23(5).


9. Tsuchiya T, Balestrini JL, Mendez J, Calle EA, Zhao L, Niklason LE. Influence of pH on extracellular matrix preservation during lung decellularization. Tissue Eng Part C Methods. 2014;20(12):1028-36.


10. Mendoza-Novelo B, Castellano L, Delgado J, Quintero-Ortega I, Vargas-Mancilla J, Vega-Gonzalez A. Decellularized laminate tissues and their derivatives as templates intended for abdominal wall regeneration. Materials Letters. 2016;164:659-64.


11. Dong X, Wei X, Yi W, Gu C, Kang X, Liu Y, et al. RGD-modified acellular bovine pericardium as a bioprosthetic scaffold for tissue engineering. Journal of Materials Science: Materials in Medicine. 2009;20(11):2327-36.


12. Grauss R, Hazekamp M, Van Vliet S, Gittenberger-de Groot A, DeRuiter M. Decellularization of rat aortic valve allografts reduces leaflet destruction and extracellular matrix remodeling. The Journal of Thoracic and Cardiovascular Surgery. 2003;126(6).


13. Liao J, Yang L, Grashow J, Sacks MS. The relation between collagen fibril kinematics and mechanical properties in the mitral valve anterior leaflet. 2007.


14. Gilbert TW, Sellaro TL, Badylak SF. Decellularization of tissues and organs. Biomaterials. 2006;27(19):3675-83.


15. Lumpkins SB, Pierre N, McFetridge PS. A mechanical evaluation of three decellularization methods in the design of a xenogeneic scaffold for tissue engineering the temporomandibular joint disc. Acta Biomater. 2008;4(4):808-16.

16. Horowitz B, Bonomo R, Prince AM, Chin SN, Brotman B, Shulman RW. Solvent/detergent-treated plasma: a virus-inactivated substitute for fresh frozen plasma. Blood. 1992;79(3):826-31.


17. Brown BN, Freund JM, Han L, Rubin JP, Reing JE, Jeffries EM, et al. Comparison of three methods for the derivation of a biologic scaffold composed of adipose tissue extracellular matrix. Tissue Eng Part C Methods. 2011;17(4):411-21.


18. Meyer SR, Chiu B, Churchill TA, Zhu L, Lakey JR, Ross DB. Comparison of aortic valve allograft decellularization techniques in the rat. J Biomed Mater Res A. 2006;79(2):254-62.


19. Prasertsung I, Kanokpanont S, Bunaprasert T, Thanakit V, Damrongsakkul S. Development of acellular dermis from porcine skin using periodic pressurized technique. J Biomed Mater Res B Appl Biomater. 2008;85(1):210-9.


20. Vavken P, Joshi S, Murray MM. TRITON-X is most effective among three decellularization agents for ACL tissue engineering. J Orthop Res. 2009;27(12):1612-8.


21. Gillies AR, Smith LR, Lieber RL, Varghese S. Method for decellularizing skeletal muscle without detergents or proteolytic enzymes. Tissue Eng Part C Methods. 2011;17(4):383-9.