Antimicrobial Effects of Cold Plasma on the Pathogenic Bacterium Salmonella enteritidis Existed on the Egg Shell
Subject Areas : Microbiology
P. Bohlouli
1
,
R. Jalalirad
2
,
D. Dorranian
3
1 - M. Sc. Student of Food Science and Technology, Science and Research Branch, Islamic Azad University,
Tehran, Iran.
2 - Assistant Professor, Pasteur Institute of Iran, Tehran, Iran.
3 - Professor of Plasma Physics Research Center, Faculty of Science, Science and Research Branch, Islamic Azad
University, Tehran, Iran.
Keywords: Antimicrobial, Cold Plasma, Egg, Microorganism, Salmonella enteritidis,
Abstract :
Introduction: Cold plasma or non-thermal plasma technology is one of the methods of foodprocessing that is used to inactivate pathogen microorganisms and improve food safety. Coldplasma can affect the inactivation of a wide range of microorganisms, without harming thehost and healthy tissues.The aim of this study was to evaluate the effect of cold plasma directly on reducing thenumbers of Salmonella enteritidis on egg shell and also to determine the effect of plasmaexposure time and the composition of the injected gas.Materials and Methods: In this study, the effect of cold atmospheric plasma radiation ofargon and argon containing 5% oxygen gas, at the rate of 3 liters / min, and at three differenttimes on inactivation of Salmonella enteritidis was investigated.Results: The effect of argon gas radiation for 1, 3 and 6 minutes on the reduction ofsalmonella enteritidis numbers being 4.490, 3.948, 0 cfu /ml respectively, was significant at1% probability level. Also, the radiation effect of argon containing 5% oxygen at 1, 3 and 6minutes,on reduction of Salmonella enteritidis numbers was 4.559, 4.226 and 0 cfu /ml,respectively. Both groups of the treatments caused a significant decreasing trend at thestatistical level of 1% as compared to the control sample.Conclusion: The obtained data indicated that the effects of the gas type, as well as theirradiation time and their interaction on the tested bacterium were statistically significant atp>0/01 as compared to the control.
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Kim, B., Yun, H., Jung, S., Jung, Y., Jung, H., Choe, W. & Jo, C. (2011). Effect of atmospheric pressure plasma on inactivation of pathogens inoculated onto bacon using two different gas compositions. Food Microbiology, 28(1), 9–13.
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Pasquali, F., Fabbri, A., Cevoli, C., Manfreda, G. & Franchini, A. (2010). Hot air treatment for surface decontamination of table eggs. Food Control, 21(4), 431–435.
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Schutz, G. E., Fawcett, H. A., Lewno, M. J., Flick E. L. & Kirby, R. S. (1996). Prevalence of Salmonella enteritidis in poultry shell eggs in Arkansas. Southern Medical Journal, 89(9), 889–891.
Shapoori, R., Rahnama, M. & Iqbalzadeh, S. H. (1394). Investigation of the prevalence of Salmonella serotypes in chicken and eggs and determining their antibiotic sensitivity in Zanjan. Zanjan University Journal of Biological Sciences, 2(3), 37–42 [In Persian].
Tendero, C., Tixier, C., Tristant, P., Desmaison, J. & Leprince, P. (2006). Atmospheric pressure plasmas: A review.
Spectrochimica Acta Part B: Atomic Spectroscopy, 61(1), 2–30.
Yang ,Y. J., Huang, M. C., Wang, S. M., Wu, J. J., Cheng, C. P. & Liu, C. C. (2002). Analysis of risk factors for bacteremia in children with nontyphoidal Salmonella gastroenteritis. European Journal of Clinical Microbiology and Infectious Diseases, 21(4), 290–293.
Afshari, R. & Hosseini, H. (2012). Atmospheric Pressure Plasma Technology: A New Tool for Food Preservation. International Conference on Environment. Energy and Biotechnology IPCBEE vol. 33.
Bárdos, L. & Baránková, H. (2010). Cold atmospheric plasma: Sources, processes, and applications. Elsevier Ltd. 6705–6713.
Colagar, A. H., Memariani, H., Sohbatzadeh, F. & Omran, A. V. (2013). Nonthermal atmospheric argon plasma jet effects on Escherichia coli biomacromolecules. Applied Biochemistry and Biotechnology, 171(7), 1617–1629.
Cooper, M., Fridman, G., Staack, D., Gutsol, A. F., Vasilets, V. N., Anandan, S., Cho, Y. I., Fridman, A. & Tsapin, A. (2009). Decontamination of surfaces from extremophile organisms using nonthermal atmospheric pressure plasmas. IEEE Transactions on Plasma Science, 37(6), 866–71.
Fernández, A. & Thompson, A. (2012). The inactivation of Salmonella by cold atmospheric plasma treatment. Elsevier Ltd. 678–684.
Fridman, G., Peddinghaus, M., Balasubramanian, M., Ayan, H., Fridman, A., Gutsol, A. & Brooks, A. (2006). Blood coagulation and living tissue sterilization by floating-electrode dielectric barrier discharge in air. Plasma Chemistry and Plasma Processing, 26(4), 425–42.
Fridman, A. A. & Kenedy, L. A. (2004). Plasma Physics and Engineering. New York. Taylor & Francis Routledge, Great Britain.
Hajipour, A. (1391). Health assessment of local eggs in Salmonella pollution in Khorramabad. Proceedings of the National Congress of Food Safety Researchers and Elites. Tehran [In Persian].
Kim, B., Yun, H., Jung, S., Jung, Y., Jung, H., Choe, W. & Jo, C. (2011). Effect of atmospheric pressure plasma on inactivation of pathogens inoculated onto bacon using two different gas compositions. Food Microbiology, 28(1), 9–13.
Moritz, M., Wiacek, C., Koethe, M. & Braun, P. G. (2017). Atmospheric pressure plasma jet treatment of Salmonella enteritidis inoculated egg shells. International Journal of Food Microbiology, 245, 22–28.
Pasquali, F., Fabbri, A., Cevoli, C., Manfreda, G. & Franchini, A. (2010). Hot air treatment for surface decontamination of table eggs. Food Control, 21(4), 431–435.
Ragni, L., Berardinelli, A., Vannini, L., Montanari, C., Sirri, F., Guerzoni, M. E. & Guarnieri, A. (2010). Non-thermal atmospheric gas plasma device for surface decontamination of shell eggs. Journal of Food Engineering, 100(1), 125–132.
Schutz, G. E., Fawcett, H. A., Lewno, M. J., Flick E. L. & Kirby, R. S. (1996). Prevalence of Salmonella enteritidis in poultry shell eggs in Arkansas. Southern Medical Journal, 89(9), 889–891.
Shapoori, R., Rahnama, M. & Iqbalzadeh, S. H. (1394). Investigation of the prevalence of Salmonella serotypes in chicken and eggs and determining their antibiotic sensitivity in Zanjan. Zanjan University Journal of Biological Sciences, 2(3), 37–42 [In Persian].
Tendero, C., Tixier, C., Tristant, P., Desmaison, J. & Leprince, P. (2006). Atmospheric pressure plasmas: A review.
Spectrochimica Acta Part B: Atomic Spectroscopy, 61(1), 2–30.
Yang ,Y. J., Huang, M. C., Wang, S. M., Wu, J. J., Cheng, C. P. & Liu, C. C. (2002). Analysis of risk factors for bacteremia in children with nontyphoidal Salmonella gastroenteritis. European Journal of Clinical Microbiology and Infectious Diseases, 21(4), 290–293.
