Molecular Detection of Methicillin Resistant Enterotoxin a Producing Staphylococcus aureus in Profiteroles in Karaj in 2016

Subject Areas : Microbiology

M. Makvandi ¹ (M. Sc. Student of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran.)
N. Harzandi ² (Assistant Professor of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran.)

Received: 2019-07-02 Accepted : 2019-07-02 Published : 2019-06-22

Keywords: Enterotoxin A, MRSA, Mec gene, Nuc gene, Profilteroles, Sea gene,

Abstract :

Introduction: Staphylococcal enterotoxins are members of a family of more than 20 different staphylococcal and streptococcal exotoxins that are functionally related and share corresponding gene sequence homology. These bacterial proteins are known to be mitogen and are responsible for significant human diseases including food poisoning. The aim of this study was to detect the enterotoxin A producing Staphylococcus aureus in profiteroles.Materials and Methods: Sampling was carried out from 50 confectionaries in Karaj from mid-August 2016 to February 2017. Serial dilutions from each sample were prepared and cultured on Baird parker agar. After 48 hours incubation at 37ºC, black colonies were selected. Following transfer to blood agar, susceptibility of isolates to oxacillin was determined using disc diffusion method and presence of nuc, mecA and sea genes was checked using PCR.Results: In total, based on culture results 8 out of 50 samples (16%) had Staphylococcus aureus contamination. PCR method results showed the presence of nuc gene in 14 (28%) of the samples, mecA gene and sea gene in 5 (10%), 3 (6%) of the samples respectively.Conclusion: Remarkable frequency of enterotoxin A producing MRSA isolates in different foods and specially profiteroles as an enrichment medium for growth and toxin production of bacteria, is a warning for public health.

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