Isolation and molecular identification of thiocyanate biodegrationbacteria in the sediments from Maharloo lake, Fars province

Subject Areas : Environmental pollutions (water, soil and air)

Fahimeh Motallebi ¹ , Farshid Kafilzadeh ²

1 - MSc., Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran.
2 - ‌Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran. *(Corresponding Authours)

Received: 2017-08-16 Accepted : 2017-10-11 Published : 2019-07-23

Keywords: PCR, Maharloo, thiocyanate, Micrococcusluteus, Bacillus sphaericus,

Abstract :

Background and Objective: Thiocyanate is an inorganic, one compound carbon and an important member of the cyanide family, which is derived from natural and industrial sources. It is largely produced by Metal and coke extraction industries. This toxic compound, causing undesirable effects in living organisms. Due to the presence of this toxic compound in the Maharloo Lake, this study was aimed to isolate and molecular identification of bacteria degrading thiocyanate from sediments of the Maharloo. Method: Sampling was performed during summer and spring of 2015 and 2016 form 5 stations. Isolation of Thiocyanate-degrading bacteria was conducted in the M9 medium. After identifying the physiological and biochemical thiocyanate degrading bacteria, MIC test, kinetics of growth and the rate of decomposition of thiocyanate were conducted. Finally, resistant bacteria were identified by PCR-based gene 16S rRNA. Findings: 9 species had the ability of thiocyanate degradation in Maharloo lake. Bacillus sphaericus and Micrococcus luteus showed the highest potential to remove thiocyanate and the highest resistance (50 grams per liter) than other bacteria. The Most thiocyanate biodegrationrate was related to B. sphaericus (66.66%) and M. luteus (50%) which showed 97% and 92% homology to Planococcus citreus strain NBRC 15849and Bacillus aerius strain 24K respectively. Discussion and Conclusion: The result of this study showed that the Maharloo lake contains powerful bacteria in thiocyanate biodegradation, so that B. sphaericus can break up to 66.66%. By providing a framework for the growth of these bacteria, they can be used to detoxify and remove thiocyanate from contaminated water.                          

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