Abstract :
Background and Objectives: One of the consequences of industrial activities is the entry of toxic compounds such as cyanide into the environment, the neglect of which threatens the health of humans and other living organisms. The present study was performed to investigate the biological removal of cyanide from wastewater and to identify resistant microorganisms.
Materials and methods: Artificial sewage Potassium by concentrations of 5, 20, 50, 100, 150 and 200 mg/l was studied in batch reactors with a capacity of 2 liters. Secondary sludge of Wastewater Treatment Plant with mixed media was used as the medium in reactor. Volatile suspended solid, total suspended solid, CN, NH3, NO3 factors were measured based on the standard method book. Rotifers, ciliates and algae were also identified using microscopy version IIS.
Results: The results showed that biological treatment can remove cyanide up to concentration of 150 ppm and higher initial MLVSS played a main role in increasing removal of cyanide from sewage. Finally, Pseudomonas cyanide, coliforms (except fecal coliforms), bacillus, fungi and also Carchesium (one of ciliates), Philodina (one of rotifers) and Oscillatoria (one of algae) identified as resistant strains to cyanide and Aspidisca, Proals and Ulothrix detected as the most sensitive rotifers, ciliates and algae, respectively.
Conclusion: Results indicated that biological treatment of activated sludge for cyanide removal from sewage is an efficient way specially when substrate include only resistant microorganisms and can be consider as viable alternative for cyanide removal instead of chemical removal methods.
References:
Ebbs, S.,2004. Biological degradation of cyanide compound. Current Opinion in Biotechnology, 2004,Vol. 15, pp.231–236.
Smith, A., Mudder, T.I.,1991. The chemistry and treatment of cyanidation Mining. Journal Books Ltd, London,United Kingdom.
Young, C.A., Jordan, T.S.,1995. Cyanide remediation:current and past technologies. Proceedings of the 10th Annual Conference on Hazardous Waste Research, Great Plains/Rocky Mountain Hazardous Substance Research Center, Kansas State University, Kansas, pp. 104-129.
Dubey, S.K., Holmes, D.S.,1995. Biological cyanide destruction mediated by microorganisms. World J Microbiol Biotechnol,Vol. 11, pp. 257-265.
Ware, G.C., Painter, H.A.,1955. Bacterial Utilization of Cyanide. Nature, 175, 900.
Brink, R.J.,1960. Biological decomposition of cyanide. Proc. 3rd. Conf. on Biological Wastes Treatment, manhattan College.
Ludzack, F.J., Schaffer, R.B.,1960. Activated sludge treatment of cyanate and thiocyanate. Proc. 15th Industial Waste Conf, Purdue Univ, pp. 439-460.
Gurnham, C.F., 1965. Cyanide destruction on trickling filters. Proc. 10th Industrial Waste Conf, Purdue Univ, pp. 186-193.
Allen, J., Strobel, G.A.,1966. The assimilation of HCN by a variety of fungi.Canadian Journal of Microbiology, Vol. 12, pp. 414-416.
10.Kostenbadner, P.D., Flecksteiner, J.W., 1969. Biological oxidation of coke plant weak ammonia liquo. J.WPCF,Vol. 41, pp.199-207.
Raef, S.F., Characklis, W.G., Kessick, M.A., Ward, C.H., 1976. Fate of Cyanide and related compounds in aerobic microbial systems II. Microbial Degradation. Water Research, Vol. 11.pp. 485-492.
12.Luthy, R.G.,1981. treatment of coal coking and coal gasification wastewatera. J. WPCF, Vol. 53, pp. 325-339.
13.Katayama, Y., Kuraishi, H.,1978. Characteristics of thiobacillus thioparus and its thiocyanate assimilation.Can. J. Microbial, Vol.24, pp.804-810.
Betts, P.M., Rinder, D.F., fleeker, J.R., 1979. Thiocyanate utilization by an arthrobacter. Can. J. Microbial, Vol. 25, pp. 1277-1282.
15.Gaudy, A.F., Gaudy, E.T., Feng, Y.J ., Brucggemann, G.,1982. treatment of cyanide waste by the Extended aeration process. J. WPCF,Vol. 54, pp.153- 164.
Mudder, T.I ., Whitlock, J.L., 1983. Biological treatment of cyanidation wastewaters. Proc. 38th Industrial waste Con, Purdue Univ, pp. 279-288.
Meyers, PR., Gokool, P., Rawlings, DE., Woods, DR., 1991. An efficient cyanidedegrading Bacillus pumilus strain. J Gen Microbiol, Vol.137, pp. 1397-1400.
Banerjee, G.,1996. Phenol and thiocyanate based wastewater treatment in RBC reactor. J. Environ. Eng, Vol. 122, pp. 941-948.
White, D.M., Schnabel, W.,1997. Treatment of cyanide waste in a sequencing bath biofilm reactor.Wat. Res, Vol. 32, pp. 254-257.
APHA.,1992. Standard Methods for the Examinations of Water and Wastewater. 18th Edn, Publication office American Public Health Association.
Akcil, A., Karahan, A.G., Ciftci, H. Sagdic, O.,2003. Biological treatment of cyanide by natural isolated bacteria (Pseudomonas sp.) Mineral Engg, Vol.16, pp. 643-649.
Gurbuz, F., Hasan, C. Akcil, A., Karahan, A.G.,2004. Microbial detoxification of cyanide solutions: a new biotechnological approach using algae, Hydrometallurgy, Vol.72, pp. 167-176 .
Navvabi Ghamsari, S. R., Haghighi, M. R., Tajrishi, M., Emtiazi, G.,1380. The effect of cyanide on the coefficients Biokinetic. 4th national conference of environmental Health, Yazd Shahid Sadoughi University of Medical Sciences and Health Services. (In Persian)
Kuyucak, N., Akcil, A., 2013. Cyanide and removal options from effluents in gold mining and metallurgical processes. Minerals Engineering, Vol. 50, pp. 13-29.
Mekuto, L., Jackson, VA., Ntwampe, SKO.,2015. Biodegradation of free cyanide and subsequent utilisation of biodegradation by-products by Bacillus consortia: optimisation using response surface methodology. Environmental Science and Pollution Research, Vol. 22 , pp. 10434–10443.
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Ebbs, S.,2004. Biological degradation of cyanide compound. Current Opinion in Biotechnology, 2004,Vol. 15, pp.231–236.
Smith, A., Mudder, T.I.,1991. The chemistry and treatment of cyanidation Mining. Journal Books Ltd, London,United Kingdom.
Young, C.A., Jordan, T.S.,1995. Cyanide remediation:current and past technologies. Proceedings of the 10th Annual Conference on Hazardous Waste Research, Great Plains/Rocky Mountain Hazardous Substance Research Center, Kansas State University, Kansas, pp. 104-129.
Dubey, S.K., Holmes, D.S.,1995. Biological cyanide destruction mediated by microorganisms. World J Microbiol Biotechnol,Vol. 11, pp. 257-265.
Ware, G.C., Painter, H.A.,1955. Bacterial Utilization of Cyanide. Nature, 175, 900.
Brink, R.J.,1960. Biological decomposition of cyanide. Proc. 3rd. Conf. on Biological Wastes Treatment, manhattan College.
Ludzack, F.J., Schaffer, R.B.,1960. Activated sludge treatment of cyanate and thiocyanate. Proc. 15th Industial Waste Conf, Purdue Univ, pp. 439-460.
Gurnham, C.F., 1965. Cyanide destruction on trickling filters. Proc. 10th Industrial Waste Conf, Purdue Univ, pp. 186-193.
Allen, J., Strobel, G.A.,1966. The assimilation of HCN by a variety of fungi.Canadian Journal of Microbiology, Vol. 12, pp. 414-416.
10.Kostenbadner, P.D., Flecksteiner, J.W., 1969. Biological oxidation of coke plant weak ammonia liquo. J.WPCF,Vol. 41, pp.199-207.
Raef, S.F., Characklis, W.G., Kessick, M.A., Ward, C.H., 1976. Fate of Cyanide and related compounds in aerobic microbial systems II. Microbial Degradation. Water Research, Vol. 11.pp. 485-492.
12.Luthy, R.G.,1981. treatment of coal coking and coal gasification wastewatera. J. WPCF, Vol. 53, pp. 325-339.
13.Katayama, Y., Kuraishi, H.,1978. Characteristics of thiobacillus thioparus and its thiocyanate assimilation.Can. J. Microbial, Vol.24, pp.804-810.
Betts, P.M., Rinder, D.F., fleeker, J.R., 1979. Thiocyanate utilization by an arthrobacter. Can. J. Microbial, Vol. 25, pp. 1277-1282.
15.Gaudy, A.F., Gaudy, E.T., Feng, Y.J ., Brucggemann, G.,1982. treatment of cyanide waste by the Extended aeration process. J. WPCF,Vol. 54, pp.153- 164.
Mudder, T.I ., Whitlock, J.L., 1983. Biological treatment of cyanidation wastewaters. Proc. 38th Industrial waste Con, Purdue Univ, pp. 279-288.
Meyers, PR., Gokool, P., Rawlings, DE., Woods, DR., 1991. An efficient cyanidedegrading Bacillus pumilus strain. J Gen Microbiol, Vol.137, pp. 1397-1400.
Banerjee, G.,1996. Phenol and thiocyanate based wastewater treatment in RBC reactor. J. Environ. Eng, Vol. 122, pp. 941-948.
White, D.M., Schnabel, W.,1997. Treatment of cyanide waste in a sequencing bath biofilm reactor.Wat. Res, Vol. 32, pp. 254-257.
APHA.,1992. Standard Methods for the Examinations of Water and Wastewater. 18th Edn, Publication office American Public Health Association.
Akcil, A., Karahan, A.G., Ciftci, H. Sagdic, O.,2003. Biological treatment of cyanide by natural isolated bacteria (Pseudomonas sp.) Mineral Engg, Vol.16, pp. 643-649.
Gurbuz, F., Hasan, C. Akcil, A., Karahan, A.G.,2004. Microbial detoxification of cyanide solutions: a new biotechnological approach using algae, Hydrometallurgy, Vol.72, pp. 167-176 .
Navvabi Ghamsari, S. R., Haghighi, M. R., Tajrishi, M., Emtiazi, G.,1380. The effect of cyanide on the coefficients Biokinetic. 4th national conference of environmental Health, Yazd Shahid Sadoughi University of Medical Sciences and Health Services. (In Persian)
Kuyucak, N., Akcil, A., 2013. Cyanide and removal options from effluents in gold mining and metallurgical processes. Minerals Engineering, Vol. 50, pp. 13-29.
Mekuto, L., Jackson, VA., Ntwampe, SKO.,2015. Biodegradation of free cyanide and subsequent utilisation of biodegradation by-products by Bacillus consortia: optimisation using response surface methodology. Environmental Science and Pollution Research, Vol. 22 , pp. 10434–10443.