Evaluation of antibacterial activity of Avicennia marina young branch and mature leaf extract in Khoor-e-Tiab, Hormozgan province

Subject Areas : environmental management

Farshid Kafilzadeh ¹ (Associate Professor, Department of Biology, Jahrom Branch, Islamic Azad University, Jahrom, Iran)
Shekoufeh Zeinali ² (- Master of Science in Microbiology, Department of Biology, Islamic Azad University, Jahrom, Iran)
Kavous Solhjoo ³ (Assistant Professor, Department of Microbiology, University of Medical Sciences, Jahrom, Iran)

Received: 2014-05-17 Accepted : 2014-10-07 Published : 2015-09-23

Keywords: Avicennia marina, Mangrove Plant, Antibacterial Effect, Khoor-e-Tiab, Bandar Abbas,

Abstract :

Introduction and objective: Many pathogens responsible for human disease have become resistant toantibiotics and therefore finding new antibacterial agents are essential. Regarding to the fact thatmangrove plant (Avicennia marina) has been used in traditional herbal medicine in south of Iran, thisstudy has been designed to identify the antibacterial effect of extract of mature leaf and young branchof the mangrove plant.Methods: The extracts of dried leaf and branch were prepared using Soxhlet extraction method andethyl acetate and methanol were used as solvents. Then the antibacterial effects of this plant werescreened by using well agar diffusion technique against few gram positive and negative bacteria, inthree different volumes of 10, 20, and 30 microliter, with concentration of 100mg/ml and the diameterof inhibitory zone was measured. Also MIC and MBC of extract of leaf and branch of mangrove plantwere measured.Results: The highest inhibitory zone (28mm) with ethyl acetate extract of both branch and leaf ofmangrove plant in volume of 30 μl, was in Shigella dysenteriae. Lowest MIC (0.4mg/ml), MBC(0.5mg/ml) with extracts of branch using ethyl acetate as solvent was shown for Bacillus subtilis.Conclusions: Results of this research has shown that extract from branch of mangrove plant usingethyl acetate as solvent has beneficial antibacterial effect against gram positive bacteria and it appearsthat plant structure of the branch has bigger active biological antimicrobial effect in compare to leaf

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