Isolation and Evaluation of Meso-philic, Thermo-philic and SRBs Bacteria and Fungi Effective on the Effluent Wastewater in the Moulding Industrials

Subject Areas : environmental management

Mohammad Hossein Habibollahi ¹ (PhD Student, Microbiological Science, Karaj Islamic Azad University.)
Mohammad Kargar ² (Assistant Professor, Faculty of Microbiological Science, Jahrom Islamic Azad University)
Abbasali Damangir ³ (PhD Student, Agricultural Science, Faculty of Research and Science, Tehran Islamic Azad University)
Sara Nabizadeh ⁴ (Msc. Farsi Language and Literature)

Received: 2010-08-11 Accepted : 2011-01-05 Published : 2014-05-22

Keywords: Bio-surfactant, Wastewater, Aerobes and Anaerobes bacteria, Moulding industrial,

Abstract :

Introduction: Nowadays due to attention to progress of environmental laws and the importance of the wastewater purification, treatment and degradation of it have been considered as one of the important subjects. The purpose of this research is isolation and evaluation of aerobes and anaerobes microorganisms effective on oil wastewater. Materials and Methods: The specimens of this research were collected from effluent wastewater containing oil compounds of moulding unit of Sarcheshmeh Copper Complex. Mesophilic, Thermophilic and SRBs bacteria and fungus were isolated in special media. Then all of the colonies were evaluated for purpose of bio-surfactant production by emulsification tests and surface tension reduction by Tensio-meter in M.S.S medium. At last the effects of colonies with the emulsification power of 2.5–4 and surface tension reduction up to 9mN/m on wastewater structure were evaluated by HPLC chromatography. Result and Discussion: Only 15 Mesophilic bacteria of 104 isolated microorganisms have shown the most effect on the wastewater structure. Thermophilic and SRBs bacteria and fungus had insignificant effects on wastewater structure. With due results of this research, omitting heavy and organic compounds from wastewater aerobe and Mesophilic bacteria should be considered more effectively.

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