Back setting of Treated Wastewater from Xanthenes Production Process into the New Fermentation Run

Subject Areas : environmental management

Afshar Alihosseini ¹ , Nurkhoda Sadeghifard ² , Narjes Sadat i Karbalaei Khom ³

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Received: 2008-05-31 Accepted : 2009-02-12 Published : 2012-04-02

Keywords: Xanthomonas campestris, Fermentation, Wastewater, xanthenes, C18 filter, back setting,

Abstract :

Parallel to development of biotechnology and fermentation processes in the country, the importance of the wastewater treatment has been increased. One of the cost effective methods in fermentation and wastewater treatment is back setting and recycling wastewater in a new run of fermentation which is used in this project to reduce xanthenes fermentation wastewater and water usage. In this project, the main chemical and microbiological characteristics of distilled wastewater were determined. Thereafter distilling was added to new batches of fermentation in different concentrations at different times; and the effect of these additions on xanthenes production quality and quantity were examined. This study showed that the use of different physic – chemical methods is necessary for the distilling treatment before its back setting in a new fermentation batch. For this purpose, in this project, the effect of treated distilling with C18 disk was examined. Further examinations showed that the back setting of distilling treated with C18 disk in 25% concentration in Xanthenes production medium leads to producing of 11.91 g/l crude Xanthenes in comparison with the positive control (culture medium prepared from tap water), 11.68 g/l crude Xanthenes, was produced. Moreover, the increase of 107% crude xanthenes, 75% pure xanthenes and 67% viscosity compared to the negative control (culture medium prepared from raw distillation) is observed.