Isolation and characterization Anthracene degrading Gram negative bacteria from water and sediment contaminanted by these compounds

Subject Areas : environmental management

Farshid Kafilzade ¹ (University of Azad, Jahrom)
Somaye behzadi shahrbabak ² (MD.Microbiology-university of Jahrom)

Received: 2009-11-19 Accepted : 2010-04-18 Published : 2014-12-22

Keywords: Anthracene-Pseudomonas-Gram ne,

Abstract :

Polycyclic aromatic hydrocarbons (PAHs)form a group of compound compused of two or more fused aromatic rings that are carcinogen and mutagenic for human and animals. thay are formed by the incomplete combustion of organic matter and fossil fuel. PAHs are released into the environment from way waste water of factories producting Coke,oil,s refineries and industrials that use from very high temperatures,also these compounds are primary substance producting cosmetics,polymers,high explosive,fertilizer,colours and etc,that transfer to environment when used. Today biodegradation of PAHs by microorganisms is the best way for fending these contamination and because bacteria have variety enzymes, hence thay are the best microorganism. Anthracene is tricyclic aromatic hydrocarbon, solid and Phenanthrene isomer. Anthracene is major source colours such Anthrakinon and Alizarin. Anthracene is formed by the incomplete combustion of fossil fuels. In this paper,we isolate anthracene degrading Gram negative bacteria from Maharlu lake.samples of lake,s sediments and water were collected from four sites in Maharlu lake. Samples filtered and cultured in Mineral medium that include inorganic compound, trace element and anthracene. Post of sequential cultures in specialized medium and employed biochemical tests, isolated gram negative bacteria and identified species and genus.The bacteria include Pseudomonas, Vibrio, Nocardia, Aeromonas, Mycobacterium isolated and characterized as anthracene degrading bacteria from lake.Among these bacteria the most bacteria was in Khoshk river,s estury and the least was in lake,s center.Whereas Pseudomonas have the most dispersal,can used as anthracene degrading indicator species.

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