Investigating and determining the antibiotic resistance pattern of Acinetobacter baumannii isolated from patients hospitalized in Tehran hospitals by PCR
Subject Areas : Microbiologyparisa Majdianfar 1 , ستاره حقیقت 2 , farshad hashemian 3 , Bahareh Nowruzi 4
1 - Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
2 - عضو هیئت علمی دانشگاه آزاد اسلامی واحد تهران پزشکی-دانشکده فناوری های نوین-گروه میکروب شناسی
3 - department of clinical pharmacy, IAUPS, tehran, Iran
4 - Department of Biotechnology, Faculty of Converging Sciences and Technologies, Islamic Azad University, Science and Research Branch, Tehran, Iran
Keywords: Acinetobacter baumannii, Antibiotic resistance, Antibiogram, PCR,
Abstract :
Abstract Aim and Background: Acinetobacter baumannii is an opportunistic bacterial pathogen responsible for a wide range of hospital-acquired infections. These bacteria take a variety of factors for resistance to different antibiotics, including resistance to β-lactams, aminoglycosides, and tetracyclines. The aims of this study were to determine the antimicrobial susceptibility pattern and prevalence of bla IMP4, bla CTX-M, tetA, and aadB genes in A. baumannii strains obtained from Imam Khomeini, Bahman, Bu-Ali, and Momenin hospitals. Material and Methods: In this cross-sectional study, 100 clinical Acinetobacter baumannii isolates were collected from various hospitals in Tehran. After the identification of Acinetobacter baumannii isolates by biochemical tests, the antibiotic susceptibility test (Kirby-Bauer method) was done according to CLSI 2021 advice against 8 antibiotics. Finally, the bla IMP4, bla CTX-M, tetA, and aadB genes were determined among the antibiotic-resistant isolates using PCR. Results: According to results, the disc diffusion results showed resistance rates of 90% for ciprofloxacin, 32% ceftazidime, 25% imipenem, 36% gentamicin, 34% streptomycin, 28% piperacillin, 5% polymyxin B and 63% tetracyclin. All isolates were susceptible to colistin. PCR results for bla IMP4, bla CTX-M, tetA, and aadB genes were detected in 63%, 62%, 76%, and 71% of resistant isolates respectively. Conclusion: This study detected clinical A. baumannii isolates harboring antibiotic resistance genes. Identification of antibiotic resistance patterns in A. baumannii and investigation of molecular epidemiology is critical to controlling the rapid spread of antimicrobial-resistant strains
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