Transcriptomics and useful techniques of defense gene expression evalution of plant
Subject Areas : botany
1 - faculty
Keywords: Real Time PCR, Transcriptomics, Northern blot, Defense gene expression, Microarray,
Abstract :
Transcriptomics is one of the most developed branches in the post-genomic era. Transcriptomics focuses on the gene expression at the RNA level and shows the genome wide information of gene structure and gene function in order to reveal the molecular mechanisms involved in specific biological processes. The plant activate the defense system after detect plant pathogens. Recently, transcriptomics has found wide application in agriculture, this knowledge are used for studing the biotic and abiotic stresses, storage diseases and the identification of resistant cultivars. It is essential the gene expression in times of stress. Transcriptomics studies of gene expression increase the information on the mechanisms of tolerance and resistance to plant disease. There are several techniques for analysis of gene expression profiles in plants. The methods used to study gene expression is placed into three categories based on hybridization techniques, techniques based on PCR and sequencing-based techniques can. In this article, it was review on methods to study gene expression and the its application in plant pathology.
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