Study of drug resistance and TEM gene existence in clinical E.Coli isolates by PCR Method
Subject Areas : biology
samaneh ghafari vosta
1
(Non-profit university of sana , sari, iran)
Keywords: TEM gene, Antibiotic resistance, PCR, Escherichia coli,
Abstract :
Background and purpose: E.Coli, ESBL producers due to their resistance to different antibiotics, has created many health problems. Therefore, in this study, we investigate on TEM gene frequency in clinical isolates of Escherichia coli with a wide range lactamase producing (ESBL) in Razi Hospital of Ghaemshahr City. Materials and methods: 1200 urine samples from inpatients and outpatients Gaemshahr Razi Hospital were studied and the final confirmation E.Coli were done by using biochemical methods. Afterwards, susceptibilities to antibiotics of different classes were determined by disc diffusion method and presence of TEM gene was checked by using PCR method and verified by sequencing. Results: The results showed that 74 isolates are E. coli. In results of Antibiogram, it was found that the most resistance of this bacteria is again antibiotics nalidixic acid with 53 samples (6/71%) and the least resistance of bacteria is again antibiotics gentamicin with 11 samples (15%). In addition, among 74 isolates of E. coli, 30 strains (5/40%) of ESBL were positive (30 of 74) and from these 30 samples, 9 samples (30%) were TEM gene (9 of 30). Conclusion: According to the results of this study, although TEM gene had no significant frequency, ESBL frequency indicates that, for treatment, prescribing antibiotics should be performed by more careful to prevent increase in strains resistant to antibiotics.
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