Neuroprotective Effect of Ghrelin on Substantia Nigra in Parkinson Disease Model Induced -MPTP
Subject Areas : biology
1 - Department of Biology, Faculty of Biological Sciences, North-Tehran Branch, Islamic Azad University, Tehran, Iran.
Keywords: Mice, IL-10, Ghrelin, TNF-α, Parkinson disease,
Abstract :
Aim and Background: Parkinson's disease is a progressive central nervous system disorder. This disease is caused by degenerative loss of dopaminergic neurons of midbrain, from substantia nigra to corpus striatum pathway. Ghrelin act as a neuroprotective against Parkinson disease, and this study aimed to investigate protective effects of ghrelin on the substantia nigra area of brain in Parkinson disease model induced- MPTP. Materials and Methods:Forty male NMRI mice were randomly divided into 5 groups of: control, saline, Parkinson, Parkinson + 0.0002 mg/kg ghrelin, and Parkinson +0.0004 mg/kg ghrelin. The Parkinson disease model was induced by MPTP intraperitoneally injection (25 mg/kg, i.p) for four days. The treatment was started one day after last MPTP inducement, by ghrelin intraperitoneally injection for 30 days. catalepsy was assessed by the means of a standard test bar. The brains were removed from the skull for histology (haematoxylin and eosin stain were used as the main principle), also tumor necrosis factor alpha (TNF-α), and Interleukin 10(IL-10) levels in substantia nigra and corpus striatum was measured using ELISA method. Results:Ghrelin effectively reduces catalepsy levels and reduces the degenerative loss of dopaminergic neurons of substantia nigra pars compacta. It will meaningfully decrease the levels of TNF-α, and positively increases the levels of IL-10 in substantia nigra and corpus striatum. Conclusion: Based on results obtained from this study, we can conclude that ghrelin has a neuroprotective role, improves catalepsy, reduces inflammatory factors, and increase anti-inflammatory factors in Parkinson disease rodent models.
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