Investigating antibiotic resistance and biofilm formation potential of Pseudomonas aeruginosa isolates isolated from Kerman hospitals

Subject Areas : microbiology

Umm Al-Banin Habili ¹ , Farokh Rokhbakhsh ²

1 - Assistant Professor, Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran
2 - Assistant Professor, Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran

Received: 2023-08-15 Accepted : 2024-07-08 Published : 2024-01-31

Keywords: Antibiotic resistance, Biofilm, Pseudomonas aeruginosa isolates, Kerman hospitals.,

Abstract :

Purpose: Pseudomonas aeruginosa is one of the causes of acquired secondary infections. This bacterium causes hospital infections, including surgical wounds, burns, and chronic lung infections. In this regard, the present study was conducted with the aim of investigating the antibiotic resistance and biofilm formation potential of Pseudomonas aeruginosa strains isolated from Kerman hospitals.

Materials and methods: This descriptive cross-sectional study was conducted on 70 hospital clinical samples. First, biochemical tests were performed on the samples in order to isolate and confirm the genus Pseudomonas aeruginosa. Antibiotic resistance pattern of strains was determined by disk diffusion method for 11 antibiotics. Then, the ability of biofilm formation in these strains was evaluated.

Findings: 29 Pseudomonas aeruginosa isolates were isolated from 70 hospital clinical samples by biochemical tests, of which 16 Pseudomonas aeruginosa strains were biofilm producers. The highest antibiotic resistance in the antibiotic resistance test is related to imipenem (17.24%), meropenem (58.27%), cefotaxime (62.06%), aztronam (75.86%), tobramycin (27.2%), respectively. 48%), cefpinem (41.37%), ciprofloxacin (34.48%), cefoxitin (48.27%), ceftazidime (48.27%), cholestin (31.03%), cefamycin (48.27%) was.

Conclusion: The results of the present study indicate an increase in the antibiotic resistance of Pseudomonas aeruginosa to the investigated antibiotics and the spread of biofilm-producing clinical isolates. Therefore, considering the clinical importance of this resistant strain in hospitals, it is necessary to quickly identify it and use appropriate infection control tools to prevent further spread of this organism.

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