Survey Of Digestive Enzymes Activity, Body Composition and Intestine Bacterial Flora Of Sobaity (Sparidentexhasta) Fingerling in Response to Different Levels Of Dietary Xylooligosaccharide
Subject Areas :Vahid Morshedi 1 , Naser Agh 2 , Jasem Marammazi 3 , Farzaneh Norii 4 , Takavar Mohammadian 5
1 - دانشجوی دکتری پژوهشکده مطالعات دریاچه ارومیه، دانشگاه ارومیه و کارشناس پژوهشی، پژوهشکده خلیج فارس دانشگاه خلیج فارس، بوشهر. ایران.
2 - دانشیار گروه شیلات پژوهشکده مطالعات دریاچه ارومیه، دانشگاه ارومیه. ایران.
3 - دانشیار پژوهشی پژوهشکده آبزی پروری جنوب کشور. اهواز. ایران.
4 - استادیار گروه شیلات پژوهشکده مطالعات دریاچه ارومیه، دانشگاه ارومیه. ارومیه. ایران.
5 - استادیار گروه بهداشت آبزیان دانشکده دامپزشکی، دانشگاه شهید چمران. اهواز. ایران.
Keywords: Intestine Bacterial Flora, body composition, Digestive enzymes, Prebiotic, Sobaity(Sparidentex,
Abstract :
Inroduction and Objective: Prebiotics, as a non-digestible food ingredient, beneficiallyaffects the host by selectively stimulating the growth, activating a limited number of bacteria, andreducing the number of harmful bacteria in the gastrointestinal tract.This study investigated the effects of xylooligosaccharide on digestive enzymes activity, body composition and intestine bacterial flora of sobaity (Sparidentex hastaMaterial and Methods:For this purpose, 405 individuals of sobaity fingerlings (45 in each replication) were prepared with an average weight of 7.64 ± 0.3 g from the Mariculture Research station of South Iranian Aquaculture Research Center. This study was carried out in a completely randomized design with three treatments and replications in fiberglass tanks with 300 liters volume. Fish were fed with feed containing 0, 0.5 and 1 percents of xylooligosaccharide for a period of 42 days. At the end of the experiment, body composition and intestine samples were collected for estimating digestive enzymes activity intestine bacterial flora and body composition. Results: The obtained results indicated that dietary xylooligosaccharide did not change sobaity digestive enzymes activity, including alkaline protease, amylse and lipase (P > 0.05). In this study, weak, positive and no significant correlation were observed between dietary prebiotic and alkaline protease activity, amylase activity and lipase activity (P > 0.05). The results of this study indicated that different levels of prebiotic did not affect body composition including protein, fat, ash and moisture and intestine bacterial flora (P > 0.05). Conclusion:Overall, this study showed that digestive enzymes activity was not affected by dietary prebiotic. Moreover, the diet supplemented with 0.5 and 1 percents of xylooligosaccharide had no significant effects on body composition and intestine bacterial flora of sobaity
