Study the Respiratory Burst Of Peripheral Blood Monocytes in the Presence Of Saccharomyces cerevisiae β–Glucan in Patients with Diabetes
Subject Areas :Mohammad Hosain Arash Asadirad 1 , Azadeh Tofighi 2 , Ali Shahnavaz 3
1 - گروه میکروبیولوژی، واحد زنجان، دانشگاه آزاد اسلامی، زنجان. ایران
2 - گروه میکروبیولوژی، واحد زنجان، دانشگاه آزاد اسلامی، زنجان. ایران
3 - گروه ریاضی و آمار، واحد زنجان، دانشگاه آزاد اسلامی، زنجان. ایران.
Keywords: Diabetes, Beta-Glucan, Saccharomyces cerevisiae, Oxidative Burst,
Abstract :
Inroduction and Objective: Diabetes mellitus is a chronic disease with epidemic potential. Diabetics are faced with complications of metabolic disorders and defects in their immune system. The use of immune system stimulating agents, such as beta-glucan is one of the researcher's interests to reduce the complications of diabetes. The present study aimed to investigate the effect of beta- glucan cell wall of Saccharomyces cerevisiae strains AT-3 on the respiratory burst of peripheral blood monocytes in patients with diabetes.Material and Methods: Peripheral blood of 53 patients with diabetes and 50 nondiabetic patients with an average of 39 years were sampled from Vali-Asr Hospital of Zanjan. Cells of Saccharomyces cerevisiae strain AT-3 were broken using sonikator. The cell wall was isolated. Beta- glucan was extracted from the wall. Nitro Blue Tetrazolium test was used to evaluate the respiratory burst of monocytes stimulated with beta glucan. Data were analyzed using multi-way ANOVA .Results:The results indicated that the respiratory burst of stimulated monocytes was increased in both men and women. The effect was dose dependent and it was significantly higher in women (pConclusion:Beta- glucan extracted fromSaccharomyces cerevisiae strain AT-3 isa potent immune system activator
