Protective Effects of Voluntary Exercise and extract of Salvia officinalis on diazinon induced Hepatotoxicity in Rats

Subject Areas :

Esmaeil Fatahi ¹ (گروه زیست شناسی، واحد آیت ا... آملی، دانشگاه آزاد اسلامی، آمل. ایران)
Akbar Hajizadeh Mogadam ² (گروه زیست شناسی، دانشگاه مازندران، بابلسر. ایران.)
Tahereh Bagery ³ (گروه فیزیولوژی ورزشی، واحد آیت ا... آملی، دانشگاه آزاد اسلامی، آمل. ایران.)

Received: 2016-11-05 Accepted : 2018-08-04 Published : 2016-10-22

Keywords: diazinon, Exercise, liver, Salvia officinalis, Hepatocytes,

Abstract :

Inroduction & Objective:Diazinon (DZN) is organophosphate pesticides which as environmental pollutants cause oxidative stress. This study was done to determinate protective effects of exercise programand Salvia officinalis extract on liver tissue damages from diazinon.Material and Methods:: in this study, 35 wistar rats randomly divided into equal groups including: control, DZN, DZN - extract, exercise- DZN, exercise- extract – DZN.  Salvia officinalis extract with 100 mg/kg dosage for 4 weeks and DZN with 200 mg/kg dosage for once via intraperitoneally injection. Animals were sacrificed 24 hours after the last injection and blood samples were collected from the heart, sera were used to measure the concentrations of ALT and AST via RIA method.   Then liver removed of body and sections were prepared for histopathological studies. Data analyzed by one-way ANOVA and Duncan.Results: Number of hepatocytes and sinusoids capillary significantly reduced in DZN group compared to control (pConclusion: Salvia officinalisextract and exercise improves the body's antioxidant status and reduces of oxidative stress against diazinon.