Investigation of genetic structure of sanjabi sheep using microsatellite markers

Subject Areas :

reza sayed sharifi ¹ (Associate Professorin Animal Science, University of Mohaghegh Ardabili, Ardabil, Iran.)
sajad Badbarin ² (Department, Kermanshah Agricultural and Natural Resources Research and Education Center, Agricultural Research Education and Extension Organization (AREEO), Kermakshah ,Iran)
nemat hedayat evrigh ³ (Associate Professorin Animal Science, University of Mohaghegh Ardabili, Ardabil, Iran 2- Assistant Professors, Animal Science)
jamal seif davati ⁴ (Associate Professorin Animal Science, University of Mohaghegh Ardabili, Ardabil, Iran 2- Assistant Professors, Animal Science)
sima savar sofla ⁵ (Agricultural Research Education and Extension Organization (AREEO), Animal Science)

Received: 2020-08-27 Accepted : 2020-09-20 Published : 2020-09-22

Keywords: Genetic structure, Microsatellite markers, sheep sanjabi,

Abstract :

Sanjabi sheep is one of the valuable breeds of sheep in the west of the country, which is very important in terms of meat and wool production. Considering the importance of native sheep breeds and their breeding in order to achieve production with more quantity and quality, identification of genetic structure and estimation of relevant parameters (number of observed and effective alleles, heterozygosity, Shannon index, etc.) The present study was performed. . The study population consisted of 100 sanjabi rams and ewes located in Mehregan station of Kermanshah province that were randomly selected. DNA extraction was performed by salt method. PCR reaction was performed using 10 microsatellite markers. Amplified DNA fragments were stained on acrylamide gel and detected by silver nitrate method. Alleles were scored according to their size and compared to the standard index PUC8 of Fermentase Company. The results of this study showed that all markers were polymorphic. The mean number of observed and effective alleles for all markers was calculated to be 4.5 and 2.9012, respectively. The highest and lowest expected heterozygosity were obtained in OarFCB11 and BMS2721 markers equal to 0.7548 and 0.5619, respectively. The expected heterozygosity for all markers (genetic diversity) was 0.6487. According to the obtained results, it can be said that the studied sheep have a desirable level of diversity and can be achieved by high breeding by breeding and selection of superior livestock.

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