In vivo murine breast cancer targeting by magnetic iron nanoparticles involved L. GG cytoplasmic fraction
Subject Areas :Salar Mokriyani 1 , Naser Harzandi 2 , Amir Tukmehchi 3 , Leila Jabalameli 4
1 - گروه میکروبیولوژی، دانشکده علوم، دانشگاه آزاد اسلامی، واحد کرج، کرج، ایران
2 - گروه میکروبیولوژی، دانشکده دامپزشکی، دانشگاه آزاد اسلامی، واحد کرج، کرج، ایران
3 - گروه میکروبیولوژی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، آذربایجان غربی، ایران
4 - گروه میکروبیولوژی، دانشکده علوم، دانشگاه آزاد اسلامی، واحد کرج، کرج، ایران
Keywords: nanoparticles, anti-cancer, in vivo, Drug delivery, Probiotic,
Abstract :
Inroduction & Objective: The use of chemical anti-cancer drugs frequently create serious side effects. However, probiotics are natural and treat different kinds of cancer without undesired effects. In this study, a nano delivery system was planned to transport the Lactobacillus rhamnosus GG (L. GG) cytoplasmic fraction (Cf) to cancerous tissue in the mouse model. Material and Methods: Magnetic iron nanoparticles (MINPs) were synthesized and loaded with L. GG-Cf (0, 0.312, 0.625, 1.25, 2.5 mg/ml) and administrated for three weeks to treat experimentally induced murine breast cancer in a constant magnetic field. At the end of the trial, the treating efficacy of this complex molecule was evaluated via western blotting and qPCR. Results: Results showed MINPS can deliver and accumulate the L. GG-Cf in cancer tissue, also the size and volume of the tumors were reduced. Additionally, in cancer tissues of treated mice with 2.5 mg/ml of Cf-MINPs significant induced apoptosis was seen compared to untreated (control), and our data proved that this induction may be due to the caspase-3 pathway. Conclusion: In conclusion, L. GG-Cf could treat the murine breast cancer and MINPs are a suitable candidate for drug delivery because of their safety, uniformity, and magnetic properties.
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