Protective Effects of Shallot Onion Against Stroke due to Middle Cerebral Artery Occlusion in Male Wistar Rats
Subject Areas :
Atefeh Payez
1
(Department of biological science, Farhangian university, Tehran, Iran)
Firoozeh Alavian
2
(Department of biological science, Farhangian university. Tehran. Iran)
Received: 2022-03-14
Accepted : 2022-05-16
Published : 2022-08-23
Keywords:
Shallot,
Rat,
MCAO,
Antioxidant,
Stroke,
Abstract :
Background and Aim: Oxidative stress is strongly involved in functional and neurological dysfunction induced by ischemia-reperfusion (IR). Therefore, approaches for using antioxidants to reverse IR-induced damage are being reviewed. Shallot is a plant with well-proven antioxidant properties; but its neuroprotective potential against cerebral ischemia has not been studied. Therefore, the present study was designed to evaluate the influence of shallot onion powder against IR-induced oxidative stress.Materials and Methods: In this experimental study, 4 groups of 7 male Wistar rats were used, which included: sham, stroke, and stroke treated with shallot onion powder and group treated with shallot onion powder. After 8 weeks of oral treatment, rats were exposed to middle cerebral artery occlusion (MCAO) for one hour. After 22 hours of reperfusion, brain activity levels of catalase (CAT), glutathione peroxidase (GPX), superoxide dismutase (SOD), and malondialdehyde (MDA) were assessed.Results: Pretreatment with shallot onion powder led to a substantial increase in the levels of CAT (P<0/001), GPX (P<0/05), SOD (P<0/01); and there was a significant reduction in MDA level (P<0/01) in shallot onion powder + MCAO groups compared to MCAO groups.Conclusion: Oral consumption of shallots significantly decreases focal ischemic brain damage/reperfusion. The defense mechanism against cerebral ischemia is linked with increased activity of antioxidants associated with lesion pathogenesis.
References:
Ghasemi, S., Alavian, K., Alavianو (2020). Nanoparticle-Based Gene Therapy Intervention for Stroke Treatment: A Systematic Review. Curr Gene Ther, 20(5);373-82.
Attarchi, S., Bakhshayesh, Eghbali B., Seyed mehdi, SM. (2020). Stroke and drivers' job fitness. Occup Med Q J, 12(3);64-74. eng.
Kuroda, S., Siesjö, (1997). Reperfusion damage following focal ischemia: pathophysiology and therapeutic windows. Clin Neurosci (New York, NY). 4(4);199-212.
Shirley, R., Ord, E.N., Work, L. (2014). Oxidative stress and the use of antioxidants in stroke. Antioxidants, 3(3);472-501.
Sivakanesan, Antioxidants for health and longevity. Molecular Basis and Emerging Strategies for Anti-aging Interventions: Springer; 2018. p. 323-41.
Christaki, S., Moschakis, T., Kyriakoudi, A., Biliaderis, C.G., Mourtzinos, Recent advances in plant essential oils and extracts: Delivery systems and potential uses as preservatives and antioxidants in cheese. Trends Food Sci Technol, 2021.
Beya, M.M., Netzel, M.E., Sultanbawa, Y., Smyth, H., Hoffman, L. (2021) Plant-based phenolic molecules as natural preservatives in comminuted meats: A review. Antioxidants,10(2);263.
Wicha, P., Tocharus, J., Janyou, A., Jittiwat, J., Changtam, C., Suksamrarn, A., et al. (2017). Hexahydrocurcumin protects against cerebral ischemia/reperfusion injury, attenuates inflammation, and improves antioxidant defenses in a rat stroke model. PloS one,12(12);
Elsayed, W.M., Abdel-Gawad, E-HA., Mesallam, D.I., El-Serafy, T. (2020).The relationship between oxidative stress and acute ischemic stroke severity and functional outcome. The Egyptian J Neurol Psychiatry Neurosurg, 56(1);1-6.
Kim, J.S., Kim, J-H. (2018). Updated molecular phylogenetic analysis, dating and biogeographical history of the lily family (Liliaceae: Liliales). Bot J Linn Soc,187(4);579-93.
Najman, K., Sadowska, A., Hallmann, (2020). Influence of thermal processing on the bioactive, antioxidant, and physicochemical properties of conventional and organic agriculture black garlic (Allium sativum L.). Appl Sci,10(23);8638.
Putnik, P., Gabrić, D., Roohinejad, S., Barba, F.J., Granato, D., Mallikarjunan, K., et al. (2019) . An overview of organosulfur compounds from Allium spp.: From processing and preservation to evaluation of their bioavailability, antimicrobial, and anti-inflammatory properties. Food chem, 276;680-91.
Asemani, Y., Zamani, N., Bayat, M., Amirghofran, (2019). Allium vegetables for possible future of cancer treatment. Phytother Res,33(12);3019-39.
Petropoulos, S.A., Di Gioia, F., Polyzos, N., Tzortzakis, N. (2020). Natural antioxidants, health effects and bioactive properties of wild Allium species. Curr pharm des, 26(16);1816-37.
Pirbalouti, A.G., Ahmadzadeh, Y., Malekpoor, (2015). Variation in antioxidant, and antibacterial activities and total phenolic content of the bulbs of mooseer (Allium hirtifolium Boiss.). Acta Agric Slov,105(1);15-22.
Asgari, S., Ansari Samani, R., Deris, F., Fard, S., Salimi, M., Mortazaei, S., et al. (2012). Antioxidant activity and the lowering effect of hydroalcoholic extract of Allium hirtifolium boisson some haemostatic factors in hypercholesterolemic rabbits. J Mazandaran Univ Med Sci, 22(91).
Nishimura, H., Higuchi, O., Tateshita, (2004). Antioxidative activity of sulfur-containing compounds in Allium species for human LDL oxidation in vitro. Biofactors. 21(1-4);277-80.
Faraji, M., Farhoodi, M., Roozbeh Nasiraie, (2018). Antibacterial Effect of Ethanolic Extract Shallot (Allium Hirtifolium) on the Clostridium botulinum & Staphylococcus aureus and Orgoanoleptic Propertise of Liquid Curd. Iran J Nutr Sci Food Technol,12(4);73-82.
Rahbar, M., Hoseini Tagavi, S., Diba, K., Haidari, (2005). In Vitro antibacterial activity of Shallot (Allium ascalonicum) crude juice. J Med Plants,4(13);26-9. eng.
Maghsoudi, A, Saeidi, (2020). Evaluation of Antimicrobial Activity of Ethanol Extract of Nine Zagros Native Medicinal Plants Against Salmonella Typhimurium. J Vet Res,75(3);380-9.
Mortazaei, S., Rafieian, M., Ansary Samani, R., Shahinfard, (2013). Comparison of phenolic compounds concentrations and antioxidant activity of eight medicinal plants. J Rafsanjan Univ Med Sci,12(7);519-30.
Siahpoosh, A., Sohangir, (2013). Phenolic Compounds and Antioxidant Activity of Methanolic Extracts of Moosir (Allium hirtifolium boiss) Bulbs. Jundishapur Sci Med J, 11(6);625-34
Alavian, F., Ghiasvand, (2018). Protective Effects of Jujube Extract against Permeability of Blood-Brain Barrier, and the Activity of Glutathione Peroxidase and Catalase in Stroke Model. Epub, 36(475);379-385.
Longa, E.Z., Weinstein, P.R., Carlson, S., Cummins, (1989). Reversible middle cerebral artery occlusion without craniectomy in rats.Stroke, 20(1);84-91.
Falahi, F., Roghani, M., Bagheri, (2010).Time-dependent hypoglycemic and hypolipidemic effect of Allium ascalonicum L. Feeding in diabetic rats. J Baboul Univ Med Sci, 12(1);16-23.
Fallahi, F., Roghani, M., Bagheri, (2009). The effect of oral feeding of Allium ascalonicum L. on thoracic aorta contractile response in diabetic rats. Koomesh. 10(3);213-8.
Alavian, F., Alavian, K., Ghiasvand. S., Rezaeian. (2020). Protective Effects of Cherry Extract on Malondialdehyde Levels, Catalase Activity, and Edema Induced by Middle Cerebral Artery Occlusion in a Rat Stroke Model. Neurosci J Shefaye Khatam, 8(3);1-9. eng.
Buege, J.A., Aust, S. [30] Microsomal lipid peroxidation. Methods in enzymology. 52: Elsevier; 1978. p. 302-10.
Kakkar, P., Das, B., Viswanathan, A modified spectrophotometric assay of superoxide dismutase. 1984.
Alavian, F., Hajizadeh, S., Bigdeli, M.R., Bayat, G.R., Javan, (2012). Evaluation of UCP2 expression in the phenomenon of ischemic resistance induced by alternating normobaric hyperoxia in a rat model of stroke. Physiol Pharmacol,16(1);54-61.
Liu, Z., Cai, Y., Zhang, X., Zhu, Z., He, (2018). High serum levels of malondialdehyde and antioxidant enzymes are associated with post-stroke anxiety. Neurol Sci, 39(6);999-1007.
Ghahremani-majd, H., Dashti, F., Dastan, D., Mumivand, H., Hadian, J., Esna-Ashari, (2012). Antioxidant and antimicrobial activities of Iranian mooseer (Allium hirtifolium Boiss) populations. HEB,53(2);116-22.
Somani, S. (1996). Exercise, drugs and tissue specific antioxidant system. Pharmacol exerc sports,32;57-95.
Vodjgani, M., Salehi, Z., Izad, (2020) .The influence of reactive oxygen species in the immune system and pathogenesis of multiple sclerosis. Autoimmune diseases.
Vakili, (2006). Mini-review for methods of induction experimental ischemia stroke: type of model, evaluation ischemic damage and neurological deficit. Koomesh, 8(1);1-10. eng.
Taati, , Alirezaei, M., Meshkatalsadat, M.H., Rasoulian, B., Dezfolian, O., Neamati, S. (2011). Antioxidant effects of aqueous fruit extract of Ziziphus jujuba on ethanol-induced oxidative stress in the liver and kidney of male rats. Sci Mag Yafte, 13(2);54-68. eng.
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Ghasemi, S., Alavian, K., Alavianو (2020). Nanoparticle-Based Gene Therapy Intervention for Stroke Treatment: A Systematic Review. Curr Gene Ther, 20(5);373-82.
Attarchi, S., Bakhshayesh, Eghbali B., Seyed mehdi, SM. (2020). Stroke and drivers' job fitness. Occup Med Q J, 12(3);64-74. eng.
Kuroda, S., Siesjö, (1997). Reperfusion damage following focal ischemia: pathophysiology and therapeutic windows. Clin Neurosci (New York, NY). 4(4);199-212.
Shirley, R., Ord, E.N., Work, L. (2014). Oxidative stress and the use of antioxidants in stroke. Antioxidants, 3(3);472-501.
Sivakanesan, Antioxidants for health and longevity. Molecular Basis and Emerging Strategies for Anti-aging Interventions: Springer; 2018. p. 323-41.
Christaki, S., Moschakis, T., Kyriakoudi, A., Biliaderis, C.G., Mourtzinos, Recent advances in plant essential oils and extracts: Delivery systems and potential uses as preservatives and antioxidants in cheese. Trends Food Sci Technol, 2021.
Beya, M.M., Netzel, M.E., Sultanbawa, Y., Smyth, H., Hoffman, L. (2021) Plant-based phenolic molecules as natural preservatives in comminuted meats: A review. Antioxidants,10(2);263.
Wicha, P., Tocharus, J., Janyou, A., Jittiwat, J., Changtam, C., Suksamrarn, A., et al. (2017). Hexahydrocurcumin protects against cerebral ischemia/reperfusion injury, attenuates inflammation, and improves antioxidant defenses in a rat stroke model. PloS one,12(12);
Elsayed, W.M., Abdel-Gawad, E-HA., Mesallam, D.I., El-Serafy, T. (2020).The relationship between oxidative stress and acute ischemic stroke severity and functional outcome. The Egyptian J Neurol Psychiatry Neurosurg, 56(1);1-6.
Kim, J.S., Kim, J-H. (2018). Updated molecular phylogenetic analysis, dating and biogeographical history of the lily family (Liliaceae: Liliales). Bot J Linn Soc,187(4);579-93.
Najman, K., Sadowska, A., Hallmann, (2020). Influence of thermal processing on the bioactive, antioxidant, and physicochemical properties of conventional and organic agriculture black garlic (Allium sativum L.). Appl Sci,10(23);8638.
Putnik, P., Gabrić, D., Roohinejad, S., Barba, F.J., Granato, D., Mallikarjunan, K., et al. (2019) . An overview of organosulfur compounds from Allium spp.: From processing and preservation to evaluation of their bioavailability, antimicrobial, and anti-inflammatory properties. Food chem, 276;680-91.
Asemani, Y., Zamani, N., Bayat, M., Amirghofran, (2019). Allium vegetables for possible future of cancer treatment. Phytother Res,33(12);3019-39.
Petropoulos, S.A., Di Gioia, F., Polyzos, N., Tzortzakis, N. (2020). Natural antioxidants, health effects and bioactive properties of wild Allium species. Curr pharm des, 26(16);1816-37.
Pirbalouti, A.G., Ahmadzadeh, Y., Malekpoor, (2015). Variation in antioxidant, and antibacterial activities and total phenolic content of the bulbs of mooseer (Allium hirtifolium Boiss.). Acta Agric Slov,105(1);15-22.
Asgari, S., Ansari Samani, R., Deris, F., Fard, S., Salimi, M., Mortazaei, S., et al. (2012). Antioxidant activity and the lowering effect of hydroalcoholic extract of Allium hirtifolium boisson some haemostatic factors in hypercholesterolemic rabbits. J Mazandaran Univ Med Sci, 22(91).
Nishimura, H., Higuchi, O., Tateshita, (2004). Antioxidative activity of sulfur-containing compounds in Allium species for human LDL oxidation in vitro. Biofactors. 21(1-4);277-80.
Faraji, M., Farhoodi, M., Roozbeh Nasiraie, (2018). Antibacterial Effect of Ethanolic Extract Shallot (Allium Hirtifolium) on the Clostridium botulinum & Staphylococcus aureus and Orgoanoleptic Propertise of Liquid Curd. Iran J Nutr Sci Food Technol,12(4);73-82.
Rahbar, M., Hoseini Tagavi, S., Diba, K., Haidari, (2005). In Vitro antibacterial activity of Shallot (Allium ascalonicum) crude juice. J Med Plants,4(13);26-9. eng.
Maghsoudi, A, Saeidi, (2020). Evaluation of Antimicrobial Activity of Ethanol Extract of Nine Zagros Native Medicinal Plants Against Salmonella Typhimurium. J Vet Res,75(3);380-9.
Mortazaei, S., Rafieian, M., Ansary Samani, R., Shahinfard, (2013). Comparison of phenolic compounds concentrations and antioxidant activity of eight medicinal plants. J Rafsanjan Univ Med Sci,12(7);519-30.
Siahpoosh, A., Sohangir, (2013). Phenolic Compounds and Antioxidant Activity of Methanolic Extracts of Moosir (Allium hirtifolium boiss) Bulbs. Jundishapur Sci Med J, 11(6);625-34
Alavian, F., Ghiasvand, (2018). Protective Effects of Jujube Extract against Permeability of Blood-Brain Barrier, and the Activity of Glutathione Peroxidase and Catalase in Stroke Model. Epub, 36(475);379-385.
Longa, E.Z., Weinstein, P.R., Carlson, S., Cummins, (1989). Reversible middle cerebral artery occlusion without craniectomy in rats.Stroke, 20(1);84-91.
Falahi, F., Roghani, M., Bagheri, (2010).Time-dependent hypoglycemic and hypolipidemic effect of Allium ascalonicum L. Feeding in diabetic rats. J Baboul Univ Med Sci, 12(1);16-23.
Fallahi, F., Roghani, M., Bagheri, (2009). The effect of oral feeding of Allium ascalonicum L. on thoracic aorta contractile response in diabetic rats. Koomesh. 10(3);213-8.
Alavian, F., Alavian, K., Ghiasvand. S., Rezaeian. (2020). Protective Effects of Cherry Extract on Malondialdehyde Levels, Catalase Activity, and Edema Induced by Middle Cerebral Artery Occlusion in a Rat Stroke Model. Neurosci J Shefaye Khatam, 8(3);1-9. eng.
Buege, J.A., Aust, S. [30] Microsomal lipid peroxidation. Methods in enzymology. 52: Elsevier; 1978. p. 302-10.
Kakkar, P., Das, B., Viswanathan, A modified spectrophotometric assay of superoxide dismutase. 1984.
Alavian, F., Hajizadeh, S., Bigdeli, M.R., Bayat, G.R., Javan, (2012). Evaluation of UCP2 expression in the phenomenon of ischemic resistance induced by alternating normobaric hyperoxia in a rat model of stroke. Physiol Pharmacol,16(1);54-61.
Liu, Z., Cai, Y., Zhang, X., Zhu, Z., He, (2018). High serum levels of malondialdehyde and antioxidant enzymes are associated with post-stroke anxiety. Neurol Sci, 39(6);999-1007.
Ghahremani-majd, H., Dashti, F., Dastan, D., Mumivand, H., Hadian, J., Esna-Ashari, (2012). Antioxidant and antimicrobial activities of Iranian mooseer (Allium hirtifolium Boiss) populations. HEB,53(2);116-22.
Somani, S. (1996). Exercise, drugs and tissue specific antioxidant system. Pharmacol exerc sports,32;57-95.
Vodjgani, M., Salehi, Z., Izad, (2020) .The influence of reactive oxygen species in the immune system and pathogenesis of multiple sclerosis. Autoimmune diseases.
Vakili, (2006). Mini-review for methods of induction experimental ischemia stroke: type of model, evaluation ischemic damage and neurological deficit. Koomesh, 8(1);1-10. eng.
Taati, , Alirezaei, M., Meshkatalsadat, M.H., Rasoulian, B., Dezfolian, O., Neamati, S. (2011). Antioxidant effects of aqueous fruit extract of Ziziphus jujuba on ethanol-induced oxidative stress in the liver and kidney of male rats. Sci Mag Yafte, 13(2);54-68. eng.
