Evaluation of Tomato Yellow Leaf Curl Virus in Bushehr Province
Subject Areas : Plant Protection
Tahereh Ziaraty
1
(Department of Plant Pathology, Jahrom Branch, Islamic Azad University, Jahrom, Iran)
Kavous Ayazpour
2
(Department of Plant Pathology, Jahrom Branch, Islamic Azad University, Jahrom, Iran)
Keywords: Bushehr, phylogenetic analysis, Mega6, sequencing, DNA extraction,
Abstract :
Tomato is one of the most important vegetables and Tomato Yellow Leaf Curl Virus (TYLCV) is one of the most important viral disease of tomato in worldwide. In order to study the situation of TYLCV in Bushehr province, sampling of tomato plants with symptoms of virus was done during winter and spring of 2013. Samples were collected from farms of Tang Eram and Dashtestan areas. After DNA extraction , a part of coat protein gene (about 550 pb) was amplified using specific primers. Amplified segments were separated via electrophoresis and sequenced. For comparison and phylogenetic analysis, the sequences Blasted in Gene bank and using CLUSTALW and Mega4 software the phylogenetic tree was drawn via maximum parsimony method. TYLCV Phylogenetic analysis showed that all isolates were divided into two clades named as A and B. In clade A isolates of Dashtestan (KP635447) and Tang Eram (KP635448) were closed to isolates of China (AY594194) and Venezuela (EF625895) and some Iranian isolates including GU076444 from (Shiraz), KC106648 and KC 582150 from (Tehran). In clade B another isolates of this study from Dashtestan was closed to Arabic countries isolates including HM590558 from Saudi Arabia, KF229721 and KF229725 from Ammanand Iranian isolates of GU076450 from Jirof. Results showed variation among Bushehr province. Due to the results of this study can conclude that there is variation among isolates of Bushehr province and requires further studies on the existence of recombinant strains in Iranian samples.
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