Reaction of some cowpea genotypes (Vigna sinensis) to Bean common mosaic virus under greenhouse condition

Subject Areas : Plant Protection

Samaneh Javanmardi ¹ ()
Sasan Ghasemi ² ()
Nooh Shahraeen ³ ()

Received: 2013-05-25 Accepted : 2013-12-21 Published : 2014-08-23

Keywords:

Abstract :

Bean common mosaic virus is widely spread in the world, and also causes functional impairment in the quality of the product. Using tolerant and resistant genotypes is one of the main approaches to the management of this disease. For this purpose, the present study was conducted in two separate sections. 20 cowpea genotypes were studied in two separate experiments, with and without infection in a randomized complete block design, with three replications in greenhouse. The infection was conducted in 2-leaf phase by mechanical inoculation and morphological evaluation was recorded 21 days after infection. ELISA test was used to determine the infection rate of genotypes. Infection of cowpea genotypes were also checked by RT-PCR test using specific BCMV primers. Results suggest amplified fragment with length of 373 bp in the infected plants. The infection rate of different genotypes was 66.6 to 80%. According to the symptoms caused by mechanical inoculation with BCMV to cowpea genotypes was scored between 0-4 which used to obtain disease severity. According the all results, the genotypes divided to hypersensitive, sensitive, semi-resistant and resistant group.

References:

Abdallah, R. 1995. An Immunocapture/Reverse Transcription/ Polymerase Chain Reaction technique detection and molecular caharacterization of Bean common mosaic virus in mixed infections. PhD. Thesis,Wsahington state University. Department of Plant Pathology.

Alegbego, M.D. & Kashina, B.D. 2001. Status of  legume viruses in Nigeria. Journal of Sustainable Agriculture, 18: 55-69.

Allison, R., Johnston, R.E. & Dougherty, W.G. 1986. The Nucleotide Sequence of the Coding Region of Tobacco Etch Virus Genomic RNA: Evidence for The Synthesis of A Single Polyprotein. Virology, 15: 154 (1): 9-20.

Ariyarathne, H. M., Coyne, D.P.,  Jung, G.,  Skroch, P.W, Vidarer, A. K. Steadman, J. R. Miklas, P. N. & Bassett,  M.J. 1999. Molecular  mapping of disease resistance genesfor halo blight, common bacterial blight, and Bean commone mosaic virus in a segregating population of common bean. Journal of American Society of Horticultural Science, 124(6): 654-662.

Asghari Ghara, S., 2008. Strains determine of Bean Common Mosaic Virus and Bean Necrotic Mosaic Virus in Mazandaran Province. Microbiology M.Sc thesis.

Bahrami Kamangar, S. 1998. Identification and relative abundance bean mosaic-producing viruses Fars Province.  M.Sc. Thesis, Shiraz University.

Baradaran, Gh. 1997. Evaluation of bean common mosaic virus (BCMV) and determine its distribution in Chenaran and Mashhad. M.Sc. Thesis. Mashhad University.

Castillo-Urquiza, G. P., Maia, F. G., Cavalho, M. G., Pinto, C. M., & Zerbini, F. M. 2006. Characterization of A Bean Rugose Mosaic Virus(BRMV) Isolate From Mias Gerais, and Yield Loss Estimate in Beans Upon Single Infection and Double Infection with BCMV. Fitopatologia Brasileira, 31(5):455-461.

Dasgupta, I. Malathi, V. G., & Mukherjee, S. K. 2003. Genetic Engineering for Virus Resistance. Current Science, 84: 341-353.

Dinant, S. Lot, H. Albouy, J., Kuziak, C., & Meyer, M. 1991. Astier Manifacier S. Archives Virology :116(1-4): 235-52.

Drijfhout, E. 1978. Genetic Interaction Between Phaseolus Vulgaris and Bean Common Mosaic Virus Resistance With Imolicetion for Srain Identification and Breeding for Resistance.Centre. Agric. Publ. Doc., Wageningen, The Netherlends.

Drijfhout, E. 1991. Bean Common Mosaic Virus, pp. 37-39, In: Compendium of Bean Diseases EDT. by Hall, R., APS Press.

Flores- Estevez, N., Acosta-Gallegos, J.A., & Silva-Rosales, L. 2003. Bean common mosaic virus and Bean common mosaic necrosis virus in mexico. Plant Disease, 87: 21-25.

Hashemi, M. 1999. Comparison of serological, biological and physicochemical characteristics of Bean commonmosaic generator Poty viruses, Bean yellow mosaic and cowpea seed borne in Fars. M.Sc. Thesis, Shiraz University.

Hormozi Nejad, M.Shiraz, M.Mozafari, j.Dorri, H.R.Shams Bakhsh, M.Rakhshandeh Ro,F. 2008. Preliminary study of Bean common mosaic virus BCMV infection and Tomato yellow leaf curl virus TYLCV in most areas. The proceedings of 18^th Iranian Plant Protection Congress,3-6- September-Hamadan, Iran, P. 342.

Ittah, M.A. 2006. Relationship between yield and some yield components in cowpea varieties infected with two cowpea potyviruses. Global Journal of Pure and Applied Sciences, 12(1): 11-17.

Jahansoz, M. R. Naghavi, M. R. & Taleyi, A. 2003. Determine the relationships between different attributes in cowpea varieties. Journal - Research of Agricultural Sciences. 1: 143-149.

Kamelmanesh, M. M., Namayandeh, A., Dorri, H. R. & Bi Hamta, M.R., 2012. Effect of Bean common mosaic virus on  seed yield, yield components and phenological phases of common bean (Phaseolus Vulgaris L.) under field conditions. Journal of Plant Breeding and Seed, 28: 39-52.

Klark, M.F. & Adams. A. N. 1977. Charateristics of The Microplate Method of Enzyme – Linked Immunosorbent Assay for the Detection of Plant Viruses. Journal of General Virology, 34: 475-483.

Luis, J. Liste, I. & Caeiro, B. 1994. Improved Conditions For Genotype Diagnosis of Astr of The Htpo Locus. Advances in Forensic Haemagenetics, 5: 336-368.

Mavric, I., Susta-Vozlic, J. 2004. Virus Diseases and Resistance to Bean Common Mosaic and Bean Common Mosaic Necrosis Potyvirus in Common Bean (Phaseolus Vulgaris L.) Acta Agriculturae Slovenica, 83: 181-190.

Miklas, P. N. Beaver, J. S. Steadman, J.R. Silbernagel, M.J. & Freytag, G. F. 1997. Registration of Three Bean Common Mosaic Virus Resistant Navy Bean Germplasms. Crop Science, 37: 1025.

Mink, G. I., Vetten, H.J., Ward, C. W., Berger, P. H., Morales, F. S., Myers, J.M., Silbernagel, M. J., & Barnett, P. W. 1994. Taxonomy and classification of legume-infecting potyviruses. Archive of virology, 139-235.

Mosahebi Mohammadi, GH. 1972. Study of Bean common mosaic virus and characterization of different strains of the virus in Iran. MS Thesis, Tehran University.

Mowat, W.P. & Dawson, M. 1987. Detection of Plant Viruses by ELISA Using Crude Sap Extracts Unfrationaded Antisera. Journal of Virological Methods, 15: 233- 247.

Nader Pur,M. Mosahebi Mohammadi, Gh. & Kohi, M. 2000. Pathotype of Vta III, Vib and necrotic strains NL-3, NL- 8, NL-5, and virus BCMNV new report on Iran. The Proceeding of 14^th Plant Protection Congress, Isfahan, Iran -September 2000.

Peyambari, M. Kohi Habibi, M. Mosahebi, Gh. H. & Izedpanah, K. 2006. Evaluation of bean common mosaic virus (BCMV) in several provinces and bean three genotypes reaction to it. Journal of Plant Protection, 25(-3): 250- 257.

Schoonhovenran, A. & Pastor – Corrales, M. A. 1994. Standard System for the Evaluation of Bean Germplasm. General Evaluation Scale for Vival Disease. CIAT Publication Series, 16-19.

Strausbaugh, C.A., Myers, J.R., Forster, R.L., & Mc clean, P.E. 2003.A quantitative method to screen common Bean plants for resistance to Bean common mosaic necrosis virus. Phytophatology, 93: 1430-1436.

Thottappilly, G. and Rossel, H.W. 1985. World – wide occurrence and distribution of virus diseases. Cowpea Research Production: 155-171.