The effects of glutamine on serum LDH and CK in rats following a session of resistance activity
Subject Areas : Journal of Physical Activity and Hormones
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Keywords: Glutamine, resistance activity, muscle damage, lactate dehydrogenase, creatine kinase,
Abstract :
Introduction: Muscle pain after resistance activities, which occurs due to the destruction of sarcolema, increases the serum levels of LDH and CK enzymes as indicators of muscle damage. The study's results have shown that the use of food supplements such as glutamine can be useful in reducing the serum indicators of muscle damage. The present study aims to determine the effect of glutamine supplementation on serum indicators of muscle damage, including the serum levels of LDH and CK enzymes following a session of resistance activity (RA).
Material & Methods: The present experimental study was carried out using an animal model in the form of a 3 group research design with a control group. To this end, 30 adult male Wistar rats (six-week-old) were kept under controlled conditions for 2 weeks and were then divided into three equal groups, including control, and resistance activity with/ without glutamine supplementation. The glutamine supplementation group received the prepared emulsion by gavage of 200 mg/kg of body weight. After five days, both experimental groups participated in a session of RA (namely, climbing a smooth ramp with 1.5 meters height and an 85° decline) with 4 sets, 5 repetitions, 30 seconds of rest between repetitions, and 2 minutes of rest between sets. The initial load was equal to 50% of the rats' body weight. One-way analysis of variance and Bonferroni's post hoc test were used at a significance level of p ≥ 0.05.
Results: The levels of CK and LDH enzymes were different in groups. Five-day glutamine supplementation before performing a session of resistance activity can cause a lower increase in the serum levels of CK and LDH enzymes (P=0.001) as serum indicators of muscle damage, which indicates the protective effect of glutamine in maintaining the integrity and structure of cell membranes.
Conclusion: Our result suggests that glutamine consumption in animal samples can reduce muscle damage after progressive strength activity. This property can be used to benefit from the possible protective effects of glutamine in maintaining the structure and integrity of the muscle fiber cell membrane and reducing fiber damage.
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