Effects of Acetamiprid and Dichlorvos residues on some blood and biotic parameters of Laboratory Mice (Balb/c)

Subject Areas : entomology and othea arthropods

Hamid Salehi Mishani ¹ (Department of Plant protection, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran)
A. R. Jalalizand ² (Plant Protection Department, Faculty of Agriculture, Islamic Azad Unversity, Isfahan (Khorasgan) Branch, Isfahan, Iran)
Mehrdad Modaresi ³ (Department of Animal Science, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran)

Received: 2021-05-24 Accepted : 2021-08-06 Published : 2021-08-23

Keywords:

Abstract :

Residues of chemical pesticides in agricultural products have always been a concern for food health experts. In this study, 15 cucumber plants were sprayed with a concentration equal to the recommended dose (25 grams per liter for Acetamiprid and 2 ml/ liter for Dichlorvos) and twice the recommended doses. After 24 hours, the residue in cucumbers twice the recommended doses was 1.5 mg/kg for Acetamiprid and 0.5 mg/kg for Dichlorvos. Then, to investigate the biochemical effect of these amounts of residues, 60 mice were selected which 20 of them were divided into four groups. Consisted of the control group and groups 1, 2 and 3. The control group received only pure water but each group of 1 and 2 received 0.5 mg/kg Dichlorvos and 1.5 mg/kg Acetamiprid, respectively. The group 3 received one dose of both pesticides in drinking water.The results showed that, these pesticides reduced the blood Albumin significantly (Control 2.96, Acetamiprid 2.00, Dichlorvos 1.86 and the mix 1.6 g/dl). The ratio of Albumin to Globulin was 0.96 for control, and 0.68, 0.06, 0.55 g/dl for groups of 1, 2, and 3 respectively. Total protein 6.06 for control, and 4.92, 4.98, 4.48 g/dl for groups of 1, 2, 3 respectively. Also the weight loss was 8.8, Dichlorvos 0.0, Acetamiprid -0.8 and Mix -0.8g and food consumption weight loss was 666.2, Dichlorvos 372.4, Acetamiprid 392.0 and Mix 356.2g and cause changes in fertility and Follicle-stimulating hormone, Luteinizing hormone levels as well as Testosterone hormone. It can be stated that adding these pesticides to drinking water of laboratory mice can cause liver and kidney disorders, immunocompromised, and primary testicular defect, which is recommended that Monitoring programs should be performed continuously to assess the presence of pesticides in food.