Prevalence of Staphylococcus aureus, Escherichia coli and Salmonella in cream sweets in Isfahan city and antibiotic resistance of the isolates

Subject Areas : Journal of Quality and Durability of Agricultural Products and Food Stuffs

Ebrahim Rahimi ¹ (Azad uni)
Najmeh Vahed dehkordi ² (PhD student in food hygiene, Faculty Veterinary Medicine, Shahrekord Islamic Azad University, Shahrekord, Iran)
mohammad amin heidarzadi ³ (PhD student in food hygiene, Faculty Veterinary Medicine, Shahrekord University, Shahrekord, Iran)

Received: 2023-01-01 Accepted : 2023-02-15 Published : 2023-02-20

Keywords: creamy sweets, damage and food poisoning, Antibiotic resistance, bacterial,

Abstract :

Escherichia coli and Salmonella are gram-negative bacteria and are indicators of faecal contamination in food, and the presence of each of them in processed foods indicates lack of hygiene, and Staphylococcus aureus is a gram-negative bacteria. It is positive that it has the highest level of poisoning. The aim of the present study is to investigate the contamination with Staphylococcus aureus, Escherichia coli and Salmonella in cream sweets sold in Isfahan city and the antibiotic resistance of the obtained isolates. In this study, 120 cream sweets from the supply centers of this product in different parts of Isfahan city were randomly sampled and transferred to the specialized food hygiene laboratory of Shahrekord Azad University, next to the ice flask, in order to avoid secondary contamination. The standard method was used to search for microorganisms. The collected data was used in SPSS version 26 statistical software and chi-square test was used for data analysis. The results showed that Escherichia coli and Salmonella contamination was negative in all samples, but Staphylococcus aureus was confirmed in 4 cases of cream sweets. The samples were the most sensitive to tetracycline, streptomycin and amikacin and the most resistant to penicillin and ampicillin. In this survey, it was determined that a high percentage of the cream sweets offered in Isfahan city are in compliance with the existing standards in the society in terms of contamination and health quality to some extent, but in order to improve the health of the society,

References:

1- Levy N, Hashiguchi TCO, Cecchini M. Food safety policies and their effectiveness to prevent foodborne diseases in catering establ-ishments: a systematic review and meta-analysis. Food Research International. 2022: 111076.

2- Khoramrooz S, Sarikhani M, Khosravani S, Farhang Falah M, Mahmoudi Y, Sharifi A. Microbial contamination determination of Cream suit,Traditional Ice Cream and Olovia in Yasuj City. Armaghane Danesh. 2015; 20(6): 37-526.

3- Oliveira D, Borges A, Simões M. Staphyl-ococcus aureus toxins and their molecular activity in infectious diseases. Toxins. 2018; 10(6):252.

4- Guo Y, Song G, Sun M, Wang J, Wang Y. Prevalence and therapies of antibiotic-resist-ance in Staphylococcus aureus. Frontiers in cellular and infection microbiology. 2020; 10:107.

5- Janda JM, Abbott SL. The changing face of the family Enterobacteriaceae (order:“Ent-erobacterales”): new members, taxonomic issues, geographic expansion, and new dis-eases and disease syndromes. Clinical Micr-obiology Reviews. 2021;34(2):20-e00174.

6- Geddes EJ, Li Z, Hergenrother PJ. An LC-MS/MS assay and complementary web-based tool to quantify and predict compound accu-mulation in E. coli. Nature protocols. 2021; 16(10):54-4833.

7- Schuetz AN. editor Emerging agents of gastroenteritis: Aeromonas, Plesiomonas, and the diarrheagenic pathotypes of Escheric-hia coli. Seminars in Diagnostic Pathology; 2019: Elsevier.

8- Ma A, Glassman H, Chui L. Characteriz-ation of Escherichia coli possessing the locus of heat resistance isolated from human cases of acute gastroenteritis. Food microbiology. 2020;88:103400.

9- Rousset F, Cui L, Siouve E, Becavin C, Depardieu F, Bikard D. Genome-wide CRI-SPR-dCas9 screens in E. coli identify esse-ntial genes and phage host factors. PLoS genetics. 2018;14(11):e1007749.

10- Fernandes MR, Sellera FP, Moura Q, Gaspar VC, Cerdeira L, Lincopan N. Inter-national high-risk clonal lineages of CTX-M-producing Escherichia coli F-ST648 in free-roaming cats, South America. Infection, Gen-etics and Evolution. 2018;66:48-51.

11- Munck N, Smith J, Bates J, Glass K, Hald T, Kirk MD. Source attribution of Salmonella in Macadamia nuts to animal and environme-ntal reservoirs in Queensland, Australia. Foo-dborne pathogens and disease. 2020; 17(5): 357-64.

12- Bjelland AM, Sandvik LM, Skarstein MM, Svendal L, Debenham JJ. Prevalence of Salmonella serovars isolated from reptiles in Norwegian zoos. Acta veterinaria scandina-vica. 2020;62(1):1-9.

13- Myintzaw P, Moran F, Jaiswal AK. Cam-pylobacteriosis, consumer's risk perception, and knowledge associated with domestic pou-ltry handling in Ireland. Journal of Food Saf-ety. 2020;40(4):e12799.

14- Ehuwa O, Jaiswal AK, Jaiswal S. Salmo-nella, food safety and food handling practi-ces. Foods. 2021;10(5):907.

15- Turgeon P, Ng V, Murray R, Nesbitt A. Forecasting the incidence of salmonellosis in seniors in Canada: A trend analysis and the potential impact of the demographic shift. PLoS One. 2018;13(11):e0208124.

16- Zhang R, Yang S, An Y, Wang Y, Lei Y, Song L. Antibiotics and antibiotic resistance genes in landfills: A review. Science of The Total Environment. 2022;806:150647.

17- Heidarzadi MA, Rahnama M, Alipoures-kandani M, Saadati D, Afsharimoghadam A. Salmonella and Escherichia coli contamina-tion in samosas presented in Sistan and Bal-uchestan province and antibiotic resistance of isolates. Food Hygiene. 2021;11(2(42)):81-90.

18- Manoharan A, Pai R, Shankar V, Thomas K, Lalitha M. Comparison of disc diffusion & E test methods with agar dilution for antim-icrobial susceptibility testing of Haemophilus influenzae. Indian Journal of Medical Rese-arch. 2003;117:81-7.

19- Zafarzadeh A, Mahfoozi A. A Study on Staphylococcus aureus and Bacillus Cereus Contamination in Pastry Products in Gorgan. Journal of Mazandaran University of Medical Sciences. 2015;25(126):143-7.

20- Dastpeiman B, Khakipour N. Evaluation Bacterial Contamination Of E. Coli And Sta-phylococcus Aureus In Cream Filled Pastries In West Tehran City. 2021.

21- Hosseini jazani N, Babazadeh H. The prevalence of enterotoxin-producing and me-thicillin-resistant Staphylococcus aureus in different types of sweets offered in raw con-fectioneries in Urmia city. Journal of Medical Sciences Studies (Medical Journal of Urmia University of Medical Sciences). 2013; 24(1).

22- Schelin J, Wallin-Carlquist N, Thorup Cohn M, Lindqvist R, Barker GC. The form-ation of Staphylococcus aureus enterotoxin in food environments and advances in risk ass-essment. Virulence. 2011;2(6):580-92.

23- Fooladi AI, Tavakoli H, Naderi A. Detec-tion of enterotoxigenic Staphylococcus aur-eus isolates in domestic dairy products. Ira-nian journal of microbiology. 2010; 2(3): 137.

24- Nikniaz Z, Mahdavi R, Jalilzadeh H, VAHED JM. Evaluation of microbial conta-mination in cream filled pastries distributed in Tabriz confectionaries. 2011.

25- Hassanzadazar H, Taami B, Abbasi Z, Aminzare M. Microbial Contamination of Cream Filled Pastries Supplied in Confec-tioneries of Zanjan, Iran. Journal of Nutrition, Fasting and Health. 2018;6(Issue):30-4.

26- Pajohi-Alamoti M, Rezaei A, Mahmoudi R. Microbial contamination of pastry cream: Evidence from Iran. Archives of hygiene sciences. 2016;5(3):207-13.

27- Asadi S, Maram ZR, Kooshk F. Evalua-tion of microbial contamination of pastry cre-am in Arak city of Iran. Journal of Food Safety and Hygiene. 2015;1(1):26-9.