Protein Profile in Wheat as Affected by Drought Stress and Nano-Chelate Potassium

Subject Areas : Journal of Crop Ecophysiology

sodabeh jahanbakhsh ¹ , Nafiseh Asghari Asghari ² , Ali Ebadi ³ , Nasibeh Tavakoli ⁴

1 - mohaghegh university
2 - M.Sc. Graduated of Agricultural Biotechnology. Faculty of Agriculture and Natural Resource, University of Mohaghegh Ardabili, Ardabil, Iran.
3 - Prof. Department of Agronomy and Plant Breading, Faculty of Agriculture and Natural Resource, University of Mohaghegh ardabili, Ardabil, Iran.
4 - Ph.D. Student of Crop Physiology. Department of Agronomy and Plant Breading, Faculty of Agriculture and Natural Rresource, University of Mohaghegh Ardabili, Ardabil, Iran.

Received: 2016-10-25 Accepted : 2017-01-25 Published : 2017-07-23

Keywords: Drought stress, Wheat, amino acid, Nano-chelated potassium, Proteomics,

Abstract :

Tolerant systems in plants including wheat are affected by several chemical factors, like nano-chelate potassium. Use of nano-chelated potassium under drought stress reduces its negative effects and increase yield. To study protein profiles of wheat under drought stress, a factorial experiment based on completely randomized design with three replications was performed. The first factore was three levels of irrigation (85%, 60% and 35% field capacity), the second factor consisted of three wheat cultivars (Zagros, Chamran and Kuhdasht) and the third factor consisted of four nano-chelated potassium concentrations (zero, 25, 45، 65 ppm). The results showed that concentrations of proline and carbohydrates which play a major role in plant defense mechanisms due to nano-chelated potassium treatment were increased. Catalase and butpolyphenol oxidase activity decreased with increasing concentrations of nano-chelate potassium, while peroxidase activity increased. The results of two dimentional electrophoretic studies showed changes of protein expression, due to the effect of nano-chelated potassium as a direct contact with the defensive system against drought stresses, such as 11, 6, 5, 19, 14 bands. Nano-chelated potassium is also associated with proteins involved in the metabolism of carbohydrates and protein and final energy production.

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