Genetic variability and differentiation of golden mullet (Liza aurata) in seashore of Mazandaran province using microsatellite molecular

Subject Areas : New Technologies in Aquaculture Development

Mohammad Behrooz ¹

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Received: 2022-05-17 Accepted : 2022-05-17 Published : 2022-04-21

Keywords: Caspian Sea, Microsatellite, Liza aurata, Keywords: Golden mullet, Population Genetic,

Abstract :

AbstractGenetic structure of golden mullet, Liza aurata, was investigated in the Ramsar and Fereidunkenar shores using 6 microsatellite markers designed for gray mullet (Mugil cephalus) and hand mullet (Mugil soiuy). Totally, 60 samples of adult golden mullet were collected from these regions. All primer sets as polymorphic loci were used to analyze the genetic variation. Analyses revealed that average of alleles (Na) per locus was 6.2 (range 3 to 9 alleles). All sampled regions contained private alleles. The average estimates of inbreeding coefficient (Fis) values of 6 microsatellites were positive. The average observed and expected heterozygosis was 0.394 and 0.743 respectively. Deviations from Hardy-Weinberg equilibrium were in all cases (P<0.001). F-statistics (Fst) and gene flow (Nm) estimates in allele frequencies were 0.078 and 2.9, respectively. Rst and Fst estimates in AMOVA indicated significant genetic differentiation among regions (P≤0.01). Genetic distance was 0.679, indicating that the genetic difference among the studied populations is pronounced. The data generated in this study provides the genetic variation and differentiation in populations of golden mullet in the southern Caspian Sea (Mazandaran province).