Accuracy rate of pregnancy detection in dairy cow by measuring the early pregnancy factor

Subject Areas : Veterinary Clinical Pathology

صمد Mosaferi ¹ , وحید Ghaffari Laleh ² , جعفر Majidi ³ , ایرج Lotfi ⁴

1 - Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University of Tabriz, Tabriz, Iran
2 - Graduated of Veterinary Medicine, Islamic Azad University of Tabriz, Tabriz, Iran
3 - Drug Applied Research Center, Tabriz University of Medical Science, Tabriz, Iran
4 - Graduated of Veterinary Medicine, Islamic Azad University of Tabriz, Tabriz, Iran

Received: 2007-04-24 Accepted : 2007-11-11 Published : 2007-08-23

Keywords: Cow, EPF, ‍Pregnancy detection,

Abstract :

Early pregnancy factor (EPF) a protein with immunosuppressive properties can be detected in the serum of most domestic animals at early pregnancy. In this study, pregnancy detection in 41 dairy cows following artificial insemination was evaluated by detecting EPF in blood serum using the rosette inhibition test (RIT). The amount of EPF activity 1-3 and 4-7 days after artificial insemination of cows was measured by RIT. Pregnancies were confirmed 45-60 days after insemination by rectal palpation. The results indicated that sensitivity, specificity, positive predictive value, negative predictive value and accuracy of this technique in detecting pregnancy 1-3 days following insemination was 88.88,  66.66,  72.72,  85.71  and  77.77 percent  respectively and RIT titers of above 8 and below 4 were seen in pregnant and non-pregnant animals at this period. The value of the above parameters were 91,  83.83,  83.33,  91  and  87 percent  respectively  4-7  days  after insemination and also RIT titers of above 8 and below 4 were achieved in pregnant and non pregnant cows during this period. The results of this study indicated that there are significant differences in RIT titers between pregnant and non-pregnant cows 1-3 and 4-7 days following artificial insemination (P<0.05). 

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