Effects of Inositol on the protoplasmic droplet detachment and motility patterns of epididymal tail sperms in the water buffalo

Subject Areas : Veterinary Clinical Pathology

A.A Kaveh ¹ , پرویز Tajik ² , حمید Ghasemzadeh_Nava ³ , کیوان Abdi ⁴ , پژمان Mirshokraie ⁵

1 - Graduated of Veterinary Reproduction & Obstetrics, Faculty of Specialized Veterinary Sciences, Islamic Azad University, Science & Research Branch, Tehran, Iran
2 - Department of Clinical Science, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
3 - Department of Clinical Science, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
4 - Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Urmiya Branch, Urmiya, Iran
5 - Department of Clinical Science, Faculty of Veterinary Medicine, University of Shahre Kord, Shahre Kord, Iran

Received: 2008-10-29 Accepted : 2009-01-19 Published : 2008-08-22

Keywords: sperm, protoplasmic droplet, Inositol, water buffalo,

Abstract :

     The purpose of this study is to determine the effects of 1Normal concentration of Inositol solution on the protoplasmic droplet detachment and motility patterns of epididymal tail sperms in the water Buffalo. Thus, 50 pairs of adult male water buffalo testicles were collected immediately alter slaughter during March to September 2007 from urmia abattoir and transported to the laboratory alongside 4ºC ice pack in 30 minutes. Epididymal tail sperms were collected by incising the hypo vascularized areas and transported to Hams F10 culture media with normal sperm pH (6.7-7.4) and containing 10% embryonic cell serum and following 15 minutes of incubation at 37oC inside the Co2 incubator, 1N solution Inositol was added to sperm containing microtubes. Eosin-nigrosin stained sperms were then prepared from the above mentioned samples at 15, 30, 45, 60, 90, 120, 180, 240 and 360 minutes and the motility patterns were investigated using the CASA software. The number of live and dead sperms and sperms with or without the proximal and distal protoplasmic droplets were counted in the stained sperms. The results obtained from the CASA software were analyzed using t-Test. The results indicated that 1N Inositol had no significant effect on sperm motility patterns but had significantly (p<0.05) increased the number of live sperms without the protoplasmic droplet at times 30, 120, 180 and 240.

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