Determining drug resistance patterns of Clostridium perfringens isolates from acute necrotic enteritis (NE) outbreaks

Subject Areas : Veterinary Clinical Pathology

حسین Nikpiran ¹ (Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran)
بهرام Shojadoost ² (Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran)
S.M Peighambari ³ (Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran)

Received: 2008-04-20 Accepted : 2008-07-17 Published : 2008-02-20

Keywords: broiler chicken, Clostridium perfringens, drug resistance patterns, necrotic enteritis,

Abstract :

     In this study, after separating 40 Clostridium perfringens isolates from affected broiler flocks, the antibacterial susceptibility test revealed 39 drug resistance patterns in which 95% of the isolates were distributed into 38 different patterns (one isolate in each pattern) and only 5% of the isolates were placed in a single pattern (two isolates in a pattern). All 40 Clostridium perfringens isolates demonstrated resistance to chloramphenicol, vancomycin and sulfamethoxazole+trimethoprime, ranging from 0 to 17.5% while tetracycline, lincomycin and neomycin sulfate had a high level of resistance from 80 to 87.5%. Also an isolate demonstrated multiple resistance to more than 14 antibacterial compounds. 

References:

1. Devriese, L.A., Daube, G.,  Hommez, J. and Haesebrouck, F. (1993): In vitro susceptibility of clostridium perfringens isolated from farm animals to growth-enhancing antibiotics. Journal of Applied Bacteriology, 75: 55-57.


2. Drouin, P. (1999): Retour  en force  de  l enterite  necrotique. Filieres  Avicoles, 78-79.


3. Dutta, G.N. and Devriese, L.A. (1981): Macrolide-Lincosamide-streptogramin resistance patterns in clostridium perfringens from animals. Antimicrob Agents chemother., 19(2): 274-8.


4. Johansson, A., Greko, C., Engstrom, B.E. and Karlsson, M. (2004): Antimicrobial susceptibility of Swedish, Norwegian and Danish isolates of clostridium perfringens from poultry, and distribution of tetracycline resistance genes. Vet. Microbiol., 99 (3-4): 251-7.


5. Jung, W.K. (1983): Susceptibility of 50 isolates of clostridium perfringens to cefotaxime, fosfomycin, penicillin G. and vancomycin; Variable tolerance for vancomycin. Chemotherapy, 29 (2): 99-103.


6. Martel, A., Devriese, L.A., Cauwerts, K., De Gussem, K., Decostere A, and Haesebrouck, F. (2004): Susceptibility of clostridium perfringens strains from broiler chickens to antibiotics and anticoccidials. Avian pathology, 33: 3-7.


7. Miller, D.A. (1998): Clostridial diseases, p. 61-68. In D. E. Swayne, J.R. Glisson, M.M. Jackwood, J.E. Pearson, W.M. Reed (ed.), A laboratory manual for the isolation and identification of avian pathogens. 4^th ed., American Association of Avian Pathologists, Pennsylvania, USA.


8. Quinn, P.J., Carter, M.E., Markey, B.K., Donnelly, W.J. and Leonard, F.C. (2002): Veterinary Microbiology and Microbial Disease. Blackwell publishing, IowaState press, USA., pp: 28-35.


9. Quinn, P.J., Carter, M.E., Markey, B. and Carter, G.R. (1994): Clinical Veterinary Microbiology. Wolfe publishing, London, UK.

10. Summanen, P., Baron, E.N., Citron, J., Strong, D.M., Wexler, H.M. and Firegold, S.M. (1993):  Wadsworth Anaerobic Bacteriology Manual. 5^th ed., Star publishing company, Belmont. California, pp: 248-288

11. Tansuphasiriu, M.W. and Sang S.K.L. (2005): Antimicrobial Resistance among clostridium perfringens solated from various sources in Thailand. Southeast.   Asian   J.  Trop.  Med.   Public   Health,   36(4): 954-61.

12. Watkins, K.L., shryock, T.R., Dearth. R.N. and Saif, Y.M. (1997):  In vitro antimicrobial susceptibility of clostridium perfringens from commercial turkey and broiler chickens origin. Vet.  Microbial., 54(2): 195-200.