Isolation of different bacteria from pneumonic lungs of cows slaughtered in Tabriz (IRAN) abattoir

Subject Areas : Veterinary Clinical Pathology

amirparviz Rezaie Saber ¹ (استادیار گروه علوم درمانگاهی، دانشکده دامپزشکی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.)

Received: 2014-07-11 Accepted : 2014-10-20 Published : 2014-08-23

Keywords: Cow, Bacteria, Pneumonic lungs, Abattoir,

Abstract :

   In this investigation, bacterial agents involved in pneumonia of slaughtered cross-bred cows in Tabriz abattoir were studied due to importance of bacterial pneumonia among Tabriz cattles. For doing so, we referred to Tabriz slaughter house every season and fifty healthy and fifty unhealthy lungs were sampled and transferred to the microbiology laboratory of Tabriz veterinary faculty, they were cultured then colonies were studied and finally the family and species of bacteria were recognized by using differential culture media. The separated bacteria from pneumonic lungs in four seasons involved 20 Streptococcus pneumoniae, 23 Escherichia coli, 18 Staphylococcus Aureus, 1 Staphylococcus epidermidis, 9 Corynebacterium pyogenes, 11 Pseudomonas aeruginosa, 48 Pasteurella haemolytica, 8 Erysipelothrix inscidiosa, 23 Rodococcus equi, 4 Nocardia farcinica, 2 Moraxella bovis, 1 Bordetella bronchiseptica, 2 Brucella bovis and 2 Haemophilus influenza. In four unhealthy lungs in fall, Pasteurella heamolytica with Bordetella bronchiseptica and from three lesioned lungs in fall, Brucella bovis with Haemophilus influenza and in three lesioned lungs in winter, Pasteurella heamolytica with Brodetella bronchiseptica were separated. The Bacteria isolated from healthy lungs consisted of 3 Staphylococcus epidermidis, 2 Pasteurella multocida, 6 Escherichia coli and 1 Nocardia farcinica. From 200 healthy lungs which were studied, 2 cases in spring, 4 cases in summer, 4 cases in fall and 2 cases in winter were isolated. It should be noted that, no bacteria was removed from primary culture of healthy and lesioned lungs in anaerobic conditions and mycoplasma culture.

References:

• · بیگی، ن. (1376). بررسی عوامل باکتریایی پنومونی گوسفندان و گاوان کشتار شده در کشتارگاه شیراز. پایان‌نامه دکترای تخصصی دامپزشکی، دانشکده دامپزشکی شیراز، صفحات: 32-22.


• · جمشیدی، ر. (1372). بررسی باکتریولوژیکی ریه‌های پاتولوژیک بز در کشتارگاه اهواز. پایان‌نامه دوره دکترای دامپزشکی، دانشکده دامپزشکی اهواز، پایان‌نامه شماره 76، صفحات: 40-35.


• · حسنی طباطبائی،  ع. و فیروزی، ر. (1380). بیماری‌های باکتریایی دام. انتشارات دانشگاه تهران، صفحات: 217-194


• · سیاری، م. (1370). بررسی پاتولوژیک (ماکروسکوپیک و میکروسکوپیک) و باکتریولوژیکی ریه گاوهای کشتار شده در کشتارگاه اهواز. پایان‌نامة دوره دکترای تخصصی دامپزشکی، دانشکده دامپزشکی شهید چمران اهواز، پایان‌نامه شمارة 6، صفحات: 23-18.


• · قادر سهمی، ع. (65-1364). جداسازی و شناسایی مایکوپلاسما آرژینی. پایان‌نامه دوره دکترای دامپزشکی، دانشکده دامپزشکی تهران، پایان‌نامه شمارة 1532، صفحات: 59-35.
  
  
  
  • · Ahuja, M. and Mathur, K. (1985). Pathological studies on bovine pneumonia in the United Arab Emirates. Indian Journal of Veterinary Medicine, 5(2): 84-87.
  
  
  • · Almedia, J. (1987). Pneumonia and bacterial infection. Journal of Microbial Saopulo, 17(3): 213- 215.
  
  
  • · Carter, M. (1973). Diagnostic procedures in veterinary microbiology. 2nd ed., USA: Spingfied, TLL inoss, pp: 208-304.
  
  
  • · Dabo, S.M., Taylor, J.D. and Confer, A.W. (2007). Pasteruella multocida and bovine respiratory disease. Journal of Animals Health Research Review, 8(2): 129-50.
  
  
  • · Deaiwis, J. (1977). The isolation of Corynebacterium pyogcn from the nasopharynx of clinically normal calves. Ceylon Veterinary Journal, 25: 18-21.
  
  
  • · Doutre, J. and Perreau, N. (1983). Revue of elevageet medicine veterinaire des pays tropicaux. Ceylon Veterinary Journal, 36: 11-14.
  
  
  • · Dyer, N., Hansen-Lardy, L., Krogh, D., Schaan, L. and Schamber, E. (2008). An outbreak of chronic pneumonia and polyarthritis syndrome caused by Mycoplasma bovis in feedlot bison. Journal of Veterinary Diagnostic Investigation, 20(3): 369-371.
  
  
  • · Gary, K. (1985). Characterization of Corynebacterium pyogenes from sheep and cows. Ceylon Veterinary Journal, 62: 805-808.
  
  
  • · Hordagoda, N. and Dealwis, J. (1981). Bacteriological studies on normal and pneumonic lungs of bovines in srilanka. Ceylon Veterinary Journal, 29(1-4): 12-13.
  
  
  • · Krysak, D.E. (2006). Chronic pneumonia and polyarthritis syndrome in a feedlot calf. Canadian Veterinary Journal. 47(10): 1019-20, 1022.
  
  
  • · Mansmann, A. (1976). Evaluation of tracheal aspiration in the horse. Journal of the American Veterinary Medical Association, 69(6): 631-633.
  
  
  • · Mckiernan, M. and Smith, J. (1984). Bacterial isolates from the trachea of clinically health dogs. Journal of American Animal Hospital, 20: 139-142.
  
  
  • · Ojo, A. (1971). A review of microbial diseases of goats in Nigeria. Journal of Animal Health Production, 19: 5-15.
  
  
  • · Ojo, A. (1976). Bovine pneumonia in Nigeria. Journal of Animal Health Production, 8: 85-89.
  
  
  • · Ribeiro, M.G., Salerno, T., Mattos-Guaraldi, A.L. and Camello, T.C. (2008). Nocardiosis: an overview and additional report of 28 cases in cattle and dogs. Review Institute Medicine Trop of sao Paulo, 50(3): 177-185.
  
  
  • · Rice, J.A., Carrasco-Medina, L., Hodgins, D.C. and Shewen, P.E. (2007). Mannheimia haemolytica and bovine respiratory disease. Journal of Animals Health Research Review, 8(2): 117-128.
  
  
  • · Upadhya, A. (1999). Studies on the pathology of bovine pneumonia. Indian Journal of Veterinary Pathology, 7: 97-98.
  
  
  • · Withely, L.O., Mahe Swaran, J.K., Weis, D.J. and Ames, T.R. (1992). Pasterurella haemolytica A, and Bovine Respiratory Disease: Pathogenesis. Journal of Veterinary Internal Medicine, 6: 11-12.
  
  
  • · Woolums, A.R., Mason, G.L., Hawkins, L.L., Brown, C.C., Williams, S.M., Gould, J.A., et al. (2004). Microbiologic findings in feedlot cattle with acute interstitial pneumonia. American Journal of Veterinary Research, 65(11): 1525-1532.