Identification of conjunctival fornix mycoflora of Equidae (horse and mule) in Urmia district
Subject Areas :
Veterinary Clinical Pathology
abdollah araghisoureh
1
(دانشگاه آزاد اسلامی، واحد ارومیه، دانشکده دامپزشکی، استادیار گروه علوم درمانگاهی، ارومیه، ایران.)
Received: 2012-11-28
Accepted : 2013-06-08
Published : 2013-05-22
Keywords:
horse,
Conjunctiva,
Mule,
Mycoflora,
Aspergilulls spp,
Abstract :
Abstract
Fungi present on normal ocular surface become pathogen in certain conditions such as corneal damage. Therefore, Knowledge of normal conjunctival mycoflora is important for prompt and proper treatment of equine keratomycosis. The objective of this study was to isolate and identify the mycoflora of conjunctiva in clinically normal equine species in Urmia, Iran. Swabs were taken from the conjunctival fornix of both eyes of horses (n=35) and mules (n=50), without external ocular inflammation. The culture samples were seeded in Sabouraud’s dextrose agar and incubated for 10 days at 25° C. Collectively, 191 isolates belonging to 13 fungal species were identified. Aspergillus was the most commonly isolated fungal genus in horses (48.63%) and mules (38.88%). The fungal species isolated are comparable with similar studies performed on horses in other countries. This is the first report of conjunctival mycoflora in mules.
References:
عراقی سوره، ع.، مخبر دزفولی، م.ر. و محمدی چورسی، م. (1392). شناسایی جدایه‌های قارچی کیسه ملتحمه چشم بزهای سالم. مجله تحقیقات دامپزشکی، سال 68، شماره 3، صفحات: 122-107.
عراقی سوره، ع.، ابراهیمی حامد، م.، محمدپور، د. و صادقی زالی، م.ح. (1391). جداسازی و شناسایی فلور قارچی ملتحمه چشم سالم در اسب عرب ایرانی. مجله پاتوبیولوژی مقایسه‌ای. سال 9، شماره 4، صفحات: 816-811.
عراقی سوره، ع. و حسن پور، و. (1391). فلور قارچی کیسه ملتحمه گوسفندان سالم نژاد قزل در شهرستان ارومیه. مجله پژوهش های بالینی دامپزشکی، سال 3، شماره 2، صفحات: 101-95.
Andrew, S.E., Brooks, D.E., Smith, P.J., Gelatt, K.N., Chmielewski, N.T. and Whittaker, C.J.G. (1998). Equine ulcerative keratomicosis: visual outcome and ocular survival in 39 cases (1987-1996). Equine Veterinary Journal, 30: 109-116.
Andrew, S.E., Nguyen, A., Jones, G.L. and Brooks, D.E. (2003). Seasonal effects on the aerobic bacterial and fungal conjunctival flora of normal thoroughbred brood mares in Florida. Veterinary Ophthalmology, 6: 45-50.
Barsotti, G., Sgorbini, M., Nardoni, S., Corazza, M. and Mancianti F. (2006). Occurrence of fungi from conjunctiva of healthy horses in Tuscany. Veterinary Research Communication, 30: 903-906.
Barbasso, E., Sforza, F., Stoppini, R. and Peruccio, C. (2005). Mycotic flora in the conjunctival fornix of horses in Northern Italy. Ippologia, 16: 21-29.
Elligott C.R., Wilkie D.A., Kuonen, V.J., Bras I.D. and Neihaus A. (2006). Primary Aspergillus and Fusarium keratitis in a Holstein cow. Veterinary Ophthalmology,9:175-178.
Grahn, B., Wolfer, J., Keller, C. and Wilcock, B. (1993). Equine keratomicosis: Clinical and laboratory findings in 23 cases. Progress in Veterinary Compendium Ophthalmology, Local, 3: 99-103.
Gaarder, J.E., Rebhun, W.C., Ball, M.A., Pattern, V., Shin, S. and Erb, H. (1998). Clinical appearances, healing patterns, risk factors, and outcomes of horses with fungal keratitis: 53 cases (1978-1996). Journal of American Veterinary Medical Association, 213: 105-112.
Johns, I.M., Baxter, K., Booler, H. Hicks C. and Menzies-Gow, N. (2011). Conjunctival bacterial and fungal flora in healthy horses in the UK. Veterinary Ophthalmology, 14: 195-199.
Ledbetter, E.C., Patten, V.H., Scarlett, J.A. and Vermeylen, F.M. (2007). In vitro susceptibility patterns of fungi associated with keratomycosis in horses of the northeastern United States: 68 cases (1987-2006). Journal of American Veterinary Medical Association, 231: 1086-1091.
Labelle, A.L., Hamor, R.E., Barger, A.M., Maddox, C.W. and Breaux, C.B. (2009). Aspergillus flavus keratomycosis in a cat treated with topical 1% voriconazole solution.Veterinary Ophthalmology, 12: 48-52.
Martín-Suárez, E.M., Galán, A., Tardón, R. and Molleda, J.M. (2007). Clinical findings and evolution of 10 cases of equine keratomycosis diagnosed in the south of Spain (Cordoba). Proceedings of the European College of Veterinary Ophthalmologists and of the European Society of Veterinary Ophthalmology.
Mancini, N., Perotti, M., Ossi, C.M., Cavallero, A., Matusˇka, S., Paganoni, G., et al. (2006). Rapid molecular identification of fungal pathogensin corneal samples from suspected keratomycosiscases. Journal of Medical Microbiology, 55: 1505-1509.
McClellan, K.A. (1997). Mucosal defense of outer layers. Survey of Ophthalmology, 42: 233-246.
Moore, C.P., Fales, W.H., Whittington, P. and Bauer, L. (1983). Bacterial and fungal isolates from equidae with ulcerative keratitis. Journal of American Veterinary Medical Association, 182: 600-603.
Myers, D.A., Isaza, R., Ben-Shlomo, G., Abbott, J. and Plummer, C.E. (2009). Fungal keratitis in a gopher tortoise (Gopherus Polyphemus). Journal of Zoo and Wildlife Medicine, 40: 579-582.
Nasisse, M.P. and Nelms, S. (1992). Equine ulcerative keratitis. Veterinary Clinic North America Equine Practice, 8: 537-555.
Quinn, P.J., Carter, M.E., Maarkey, B.M. and Carter, G.R. (1994). Clinical Veterinary Microbiology. UK: Wolfe, pp: 213-227.
Qualls, C.W., Chandler, F.W., Kaplan, W., Breitschwerdt, E.B. and Cho, D.Y. (1985). Mycotic keratitis in a dog: concurrent Aspergillus sp. and Curvularia sp. infections. Journal of American Veterinary Medicine Association,186: 975-976.
Rosa, M., Cardozo, L.M., Pereira, J.S., Brooks, D.E., Martins, A.L.B., Florido, P.S.S., et al. (2003). Fungal flora of normal eyes of healthy horses from the State of Rio de Janeiro. Brazilian Veterinary Ophthalmology, 6(1): 51-55.
Samuelson, D.A., Andresen, T.L. and Gwin, R.M. (1984). Conjunctival fungal flora in horses, cattle, dogs, and cats. Journal of American Veterinary Medical Association, 184: 1240-1242.
Sousa, M.E., Araujo, M.A.S., Mota, R.A., Porto, W.J.N., Souza, A.K.P., Santos, J.L., et al. (2011). Fungal Microbiota from ocular conjunctiva of clinically healthy horses belonging to the military police cavalry of alagos. Brazilian Journal of Microbiology, 42: 1151-1155.
Shenoy, R., Shenoy, U.A.K. and AL Mahrooqui, Z.H. (2003). Keratomycosis due to Trichophyton mentagorphytes. Mycoses, 46: 157-158.
Stoppini, R., Barbasso, E., Peruccio, C., Ratto, A. and Gallo, G., (2003). Cheratomicosi equina in Italia Settentrionale:13 casi clinici (1998–2002). Ippologia, 4: 13-28.
Wada, S., Hobo, S. and Niwa, H. (2010). Ulcerative keratitis in thoroughbred racehorses in Japan from 1997 to 2008. Veterinary Ophthalmology, 13: 99-105.
