The Effect of Eight Weeks of Interval Training with Grape Seed Nanoparticles Supplementation on Bax and Bcl2 Gene Expression in the Heart Tissue of Myocardial Infarction Rats

Subject Areas : Open Access

Hamid Mohammadi Hosseinabadi ¹ (Department of Physical Education and Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran)
Khosro Jalali Dehkordi ² (Department of Physical Education and Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran)
gholamreza sharifi ³ (Department of Physical Education and Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran)
Zohreh Mazaheri Tirani ⁴ (Basic Medical Science Research Center, Histogenotech Company, Tehran, Iran)

Received: 2021-08-15 Accepted : 2021-09-03 Published : 2021-05-22

Keywords: Aerobic training, Myocardial Infarction, BAX, BCL2, Grape Seed,

Abstract :

Introduction: The use of exercise training and herbal supplements to treat diseases and metabolic disorders has become common among people. Considering the health-improving effects of aerobic training and grape seed nanoparticles extract, the present study was performed to investigate the effect of eight weeks of aerobic training with grape seed extract supplementation on Bax and Bcl2 gene expression in the heart tissue of rats with myocardial infarction.Materials and methods: In this experimental study, 20 rats were divided into four groups of five animals, including: 1) control, 2) aerobic training, 3) grape extract, and 4) training + grape extract. To investigate the effects of myocardial infarction on the study variables, five healthy rats were placed in the healthy control group. For eight weeks, groups 3 and 4 consumed 150 mg of grape extract daily by gavage. Also, groups 2 and 4 performed aerobic interval training, with 7 interval alternations, each including 4 minutes with an intensity of 80-90% VO2max and 3 minutes with an intensity of 65-75% VO2max. The Kolmogorov- Smirnov and one-way ANOVA with Tukey’s post- hoc tests were used to analyze the findings (P≥0.05).Findings: Induction of myocardial infarction had a significant effect on increasing Bax gene expression and decreasing Bcl2 gene expression in heart tissue (P≥0.001). However, aerobic training along with grape seed nanoparticle extract consumption had a significant effect on reducing Bax gene expression and increasing Bcl2 gene expression in the heart tissue of rats with myocardial infarction (P≥0.01).Conclusion: It seems that aerobic training along with grape extract consumption has more favorable effects on improving Bax and Bcl2 gene levels in myocardial infarction than either alone.

References:

1. Iemitsu M, Maeda S, Jesmin S, Otsuki T, Kasuya Y, Miyauchi T. Activation pattern of MAPK signaling in the hearts of trained and untrained rats following a single bout of exercise. J Appl Physiol. 2006; 101 (1): 151- 63. [DOI:10.1152/japplphysiol.00392.2005] [PMID:16484365]


2. Galang N, Sasaki H, Maulik N. Apoptotic cell death during ischemia/reperfusion and its attenuation by antioxidant therapy. Toxicology. 2000; 148 (2-3): 111- 8. [DOI:10.1016/s0300-483x(00)00201-8] [PMID:10962129]


3. Pendergrass KD, Varghese ST, Maiellaro-Rafferty K, Brown ME, Taylor WR, Davis ME. Temporal effects of catalase overexpression on healing after myocardial infarction. Circulation. 2011; 4 (1): 98- 106. [DOI:10.1161/CIRCHEARTFAILURE.110.957712] [PMCID:PMC3076122]


4. Takemura G, Kanoh M, Minatoguchi S, Fujiwara H. Cardiomyocyte apoptosis in the failing heart—a critical review from definition and classification of cell death. Int J Cardiol. 2013; 167 (6): 2373- 86. [DOI:10.1016/j.ijcard.2013.01.163] [PMID:23498286]


5. Gross A. BCL-2 family proteins as regulators of mitochondria metabolism. Biochimica et Biophysica Acta. 2016; 1857 (8): 1243- 6. [DOI:10.1016/j.bbabio.2016.01.017] [PMID:26827940]


6. Lee S-D, Kuo W-W, Wu C-H, Lin Y-M, Lin JA, Lu M-C, et al. Effects of short-and long-term hypobaric hypoxia on Bcl2 family in rat heart. Int J Cardiol. 2006; 108 (3): 376- 84. [DOI:10.1016/j.ijcard.2005.05.046] [PMID:16005992]


7. Peterson JM, Bryner RW, Sindler A, Frisbee JC, Alway SE. Mitochondrial apoptotic signaling is elevated in cardiac but not skeletal muscle in the obese Zucker rat and is reduced with aerobic exercise. J Appl Physiol. 2008; 105 (6): 1934- 43. [DOI:10.1152/japplphysiol.00037.2008] [PMCID:PMC2612474]


8. Favaloro B, Allocati N, Graziano V, Di Ilio C, De Laurenzi V. Role of apoptosis in disease. Aging. 2012; 4 (5): 330. [DOI:10.18632/aging.100459] [PMCID:PMC3384434]


9. Tekin D, Xi L, Kukreja RC. Genetic deletion of fas receptors or Fas ligands does not reduce infarct size after acute global ischemia-reperfusion in isolated mouse heart. Cell Biochem Biophys. 2006; 44 (1): 111-7. [DOI:10.1385/CBB:44:1:111] [PMID:16456239]


10. Jafari A, Pourrazi H, Nikookheslat S, Baradaran B. Effect of exercise training on Bcl-2 and bax gene expression in the rat heart. Gene Cell Tissue. 2015; 2 (4): e60174. [DOI:10.17795/gct-32833]


11. Cury-Boaventura MF, Levada-Pires AC, Folador A, Gorjão R, Alba-Loureiro TC, Hirabara SM, et al. Effects of exercise on leukocyte death: prevention by hydrolyzed whey protein enriched with glutamine dipeptide. Eur J Appl Physiol. 2008; 103 (3): 289- 94. [DOI:10.1007/s00421-008-0702-1] [PMID:18320208]


12. McMillan EM, Graham DA, Rush JW, Quadrilatero J. Decreased DNA fragmentation and apoptotic signaling in soleus muscle of hypertensive rats following 6 weeks of treadmill training. J Appl Physiol. 2012; 113 (7): 1048- 57. [DOI:10.1152/japplphysiol.00290.2012] [PMID:22858629]


13. Du Y, Guo H, Lou H. Grape seed polyphenols protect cardiac cells from apoptosis via induction of endogenous antioxidant enzymes. J Agricul Food Chem. 2007; 55 (5): 1695- 701. [DOI:10.1021/jf063071b] [PMID:17295515]


14. Zhang F-l, Gao H-q, Wu J-m, Ma Y-b, You B-a, Li B-y, et al. Selective inhibition by grape seed proanthocyanidin extracts of cell adhesion molecule expression induced by advanced glycation end products in endothelial cells. J Cardiovas Pharmacol. 2006; 48 (2): 47- 53. [DOI:10.1097/01.fjc.0000242058.72471.0c] [PMID:16954821]


15. Høydal MA, Wisløff U, Kemi OJ, Ellingsen Ø. Running speed and maximal oxygen uptake in rats and mice: practical implications for exercise training. Eur J Cardiovas Prev Rehabil. 2007; 14 (6): 753- 60. [DOI:10.1097/HJR.0b013e3281eacef1] [PMID:18043295]


16. Jiang H-K, Wang Y-H, Sun L, He X, Zhao M, Feng Z-H, et al. Aerobic interval training attenuates mitochondrial dysfunction in rats post-myocardial infarction: roles of mitochondrial network dynamics. Int J Mol Sci. 2014; 15 (4): 5304- 22. [DOI:10.3390/ijms15045304] [PMCID:PMC4013565]


17. Llópiz N, Puiggròs F, Céspedes E, Arola L, Ardévol A, Bladé C, et al. Antigenotoxic effect of grape seed procyanidin extract in Fao cells submitted to oxidative stress. J Agricul Food Chem. 2004; 52 (5): 1083- 7. [DOI:10.1021/jf0350313] [PMID:14995102]


18. Ghajari H, Hosseini SA, Farsi S. The effect of endurance training along with cadmium consumption on Bcl-2 and Bax gene expressions in heart tissue of rats. Ann Mil Health Sci Res. 2019; 17 (1): e86795. [DOI:10.5812/amh.86795]


19. Kazior Z, Willis SJ, Moberg M, Apró W, Calbet JA, Holmberg H-C, et al. Endurance exercise enhances the effect of strength training on muscle fiber size and protein expression of Akt and mTOR. PloS One. 2016; 11 (2): e0149082. [DOI:10.1371/journal.pone.0149082] [PMCID:PMC4757413]


20. Marzetti E, Calvani R, Bernabei R, Leeuwenburgh C. Apoptosis in skeletal myocytes: a potential target for interventions against sarcopenia and physical frailty–a mini-review. Gerontol. 2012; 58 (2): 99- 106. [DOI:10.1159/000330064] [PMCID:PMC7077073]


21. Koçtürk S, Kayatekin B, Resmi H, Açıkgöz O, Kaynak C, Özer E. The apoptotic response to strenuous exercise of the gastrocnemius and solues muscle fibers in rats. Eur J Appl Physiol. 2008; 102 (5): 515- 24. [DOI:10.1007/s00421-007-0612-7] [PMID:18030491]


22. Soberanes S, Panduri V, Mutlu GM, Ghio A, Bundinger GS, Kamp DW. p53 mediates particulate matter–induced alveolar epithelial cell mitochondria-regulated apoptosis. Am J Respiratory Critical Care Med. 2006; 174 (11): 1229- 38. [DOI:10.1164/rccm.200602-203OC] [PMCID:PMC2648105]


23. Razavi M, Matin H, Azarbayjani MA. Effects of concurrent regular aerobic training and garlic extract on cardiac tissue apoptosis markers in aged rats with chronic kidney disease. J Med Plant. 2017. 16 (62): 46- 54. http://jmp.ir/article-1-1344-en.html


24. Kwak H-B. Effects of aging and exercise training on apoptosis in the heart. J Exer Rehabil. 2013; 9 (2): 212. [DOI:10.12965/jer.130002] [PMCID:PMC3836520]


25. Li F, Shi W, Zhao EY, Geng X, Li X, Peng C, et al. Enhanced apoptosis from early physical exercise rehabilitation following ischemic stroke. J Neurosci Rese. 2017; 95 (4): 1017- 24. [DOI:10.1002/jnr.23890] [PMID:27571707]


26. Bagchi D, Bagchi M, Stohs SJ, Das DK, Ray SD, Kuszynski CA, et al. Free radicals and grape seed proanthocyanidin extract: importance in human health and disease prevention. Toxicol. 2000; 148 (2-3): 187- 97. [DOI:10.1016/s0300-483x(00)00210-9] [PMID:10962138]


27. Pataki T, Bak I, Kovacs P, Bagchi D, Das DK, Tosaki A. Grape seed proanthocyanidins improved cardiac recovery during reperfusion after ischemia in isolated rat hearts. Am J Clin Nutr. 2002; 75 (5): 894- 9. [DOI:10.1093/ajcn/75.5.894] [PMID:11976164]