The effect of mycorrhiza fungus and different levels of humic acid and salicylic acid on physiological characteristics and essential oil content in three cultivars of Melissa Officinalis L.

Subject Areas : Genetic

Mehdi Hamzeh Mohamadabadi ¹ , alireza ladanmoghadam ² , Elham Danaee ³ , Vahid Abdossi ⁴

1 - Department of Horticultural Sciences, Aliabad katoul Branch, Islamic Azad University, Aliabad katoul, Iran
2 - Department of Horticultural Sciences, Garmsar Branch, Islamic Azad University, Garmsar, Iran
3 - Department of Horticultural Sciences, Garmsar Branch, Islamic Azad University, Garmsar, Iran
4 - Department of Horticulture and Agronomy, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran

Received: 2021-04-11 Accepted : 2021-06-24 Published : 2024-07-19

Keywords: Salicylic acid, Humic acid, Mycorrhiza, Lemon Balm, Essential oil,

Abstract :

This study was performed to investigate the effect of mycorrhiza fungus and different levels of humic acid (zero, 200 and 400 mg/l) and salicylic acid (zero, 100 and 200 mg/l) on physiological characteristics and essential oil content in three cultivars Lemonella, Citronella and Varigated. Lemon balm (Melissa Officinalis L.) was implemented in 1397 in a greenhouse in northern Iran. The experiment was performed as a factorial in a completely randomized statistical design with 3 replications. Mycorrhiza and humic acid treatments were applied at the time of seed sowing and salicylic acid was sprayed in two stages (second and third weeks of pot cultivation). The results of this experiment showed that all treatments had a significant effect on the measured variables. The highest proline content was obtained in Citronella cultivar and control treatment and The highest phenol, carbohydrate, essential oil percentage in the treatment of mycorrhiza fungus+ humic acid 400 mg/l + salicylic acid 100 mg/l were obtained in Varigated, Citronella and Lemonella cultivars, respectively. Also the highest amount of flavonoids in the treatment of mycorrhiza fungus+ humic acid 400 mg/l + salicylic acid 200 mg/l in Citronella cultivar and the highest total antioxidant activity in the treatment of mycorrhiza fungus+ humic acid 200 mg/l + salicylic 200 mg/l in Lemonella cultivar was observed. In general, the results of this experiment showed that the application of mycorrhiza fungus with humic acid 400 mg/l and salicylic acid 100 mg/l in Lemonella, Citronella and Varigated cultivars improved the physiological characteristics and essential oil of Lemon balm.