Extraction and Identification of flavon from Artemisia turanica Krasch the extract which has been collected from Esfarayen, Khorasan province

Subject Areas : Phytochemistry

Sara Safari ¹ (Department of Phytochemistry and Essential Oils Technology, Faculty of Pharmaceutical Chemistry, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran - Iran (IAUPS))
maboubeh Taherkhani ² (Department of Chemistry, College of Science, Takestan Branch, Islamic Azad University, Takestan, Iran)

Received: 2017-11-09 Accepted : 2019-03-07 Published : 2018-05-22

Keywords: Flavonoid, Extract, Chromatography, Artemisia turanica Krasch, Esfarayen, Flavone,

Abstract :

Many researches have been conducted on identification and extraction of natural compounds from Artemisia such as monoterpenes, sesquiterpens, diterpenes, flavonoids, phenolic with the biological and pharmaceutical properties. Due to the importance of this genus, the purpose of the present study is extraction, purification and structure elucidation of a natural compound. This compound was isolated from aerial part of Artemisia turanicaKrasch. in Esfarayen at full flowering stage. So the extract obtained by maceration method and evaporated to dryness. The dried extract defatted for removing the hydrocarbons with long chains and separated by column chromatography over silica gel. All fractions were investigated by thin layer chromatography to determine the presence of natural compounds. Then, the natural compound was identified using spectrometric methods (IR, 1HNMR and 13CNMR). The results led to the identification of a flavon called 3,5,3́,4́-tetrahydroxy, 7,5́-methoxy flavones, for the first time in this plant. Therefore, it is anticipated that the plant has high antioxidant effects due to the presence of flavonoids in the plant extract.

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