Evaluation of growth adapter effect on callus induction of Momordica charantia L.

Subject Areas : Medicinal Plants

Roghieh Asgharzadeh ¹ , Husain Moradi ² , GhorbanAli Nematzadeh ³ , Ali Ghanbari ⁴

1 - 1MA, Institute of Genetics and Complex of agricultural biotechnology, University of Agricultural Sciences and Natural Resources of Sari, Mazandaran, Iran
2 - 2Assistant Professor, University of Agricultural Sciences and Natural Resources of Sari, Mazandaran, Iran
3 - 3Professor, Institute of Genetics and Complex Agricultural Biotechnology, Agricultural Sciences and Natural Resources University, Sari, Iran
4 - 4MA, University of Agricultural Sciences and Natural Resources of Sari, Mazandaran, Iran

Received: 2016-02-21 Accepted : 2016-09-11 Published : 2016-12-01

Keywords: Tissue culture, Callus Induction, Momordi cacharantia L,

Abstract :

Momordica charantia L. (Bitter melon) is a perennial  tropical  plant  belongs to Cucurbitaceae family , in rich of iron, phosphorus , vitamins A and C  which has an  economic importance, but according to  limitations dispersal  of this plant in Iran, tissue culture technique is suitable for the mass production of plant seedlings. In this research, the seeds of plant were collected from Zabol area in 2013 and the factorial experiment were done in a completely randomized design at three replications with three factors including explants type (leaves, inter nodes, nodes plus internodes) and phytohormone concentrations (2, 4-D at concentrations of 0, 0.25, 0.5 mg per liter and Benzyl adenine (BA) with concentrations of 0.25, 0.5 and 1 mg per liter), were done in GABIT Tissue culture lab. After three months the length, width, height, percentage of callus, callus color, fresh weight and dry weight were measured, respectively. Variance analysis were showed that, all characteristics in different concentration of BA, 2, 4-D and type of explants and their interaction were observed significant (P<1%). So the maximum width, length, fresh weight and dry weight of callus observed in combination treatment BA (1 mg/l) with a 2, 4-D (0.25 mg/l), interaction between hormone (0.5 mg/l) BA and (0.25 mg/l) 2, 4-D. on leaf explants callus height had a better effect than other factors. Also all concentrations of 2, 4-D and BA had 100 % callus induction in leaf explants, and the most callus have been green and yellow.  

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