Evaluation of flavonoids and phenolic compounds, antioxidant and antibacterial activity of Hypericum perforatum L. Collected from two sites in North Country

Subject Areas : Phytochemistry

hadi koohsari ¹ (Assistant Professor, Department of Microbiology, Azadshahr branch, Islamic Azad University, Azadshahr, Golestan province, Iran)
hamide khormali ² (Graduated student, Department of Food Science and technology, Azadshahr branch, Islamic Azad University, Azadshahr, Golestan province, Iran)
ayeshe khormali ³ (Young Researchers and Elite Club, Azadshahr Branch, Islamic Azad university, Azadshahr, Iran)

Received: 2017-03-08 Accepted : 2017-04-30 Published : 2017-05-22

Keywords: Mazandaran Province, antibacterial, Antioxidant, Hypericum perforatum L, Total flavonoids and phenolic,

Abstract :

This study was carried out to evaluate the phytochemical, antioxidant and antibacterial activity of Hypericum perforatum L., the aerial parts of plant were collected from two sites in Mazandaran province (Farahabad Sari and Pashakola Amol) during July to October 2015. Total phenol and flavonoid contents were determined by colorimetric method using the Folin-Ciocalteu reagent and aluminum chloride respectively. The antioxidant activity was measured by DPPH method and then the antibacterial activity was performed by agar well diffusion, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of plant extract were determined by broth micro dilution tube method. The results were showed that the total phenolic and flavonoid content and then the antioxidant capacity of plant from Sari region was more than in Amol region. The  MIC values which obtained fromSari  against Staphylococcus aureus, Bacillus cereus, Shigella dysentery and E. coli were 12, 12, 190 and 390 µg/ml respectively, while these values from Amol were 24, 24, 780 and 1560 µg/ml respectively, which results were also confirmed in agar well diffusion method and then in conclusion the plant extract from Sari region had the highest content of secondary metabolites , antioxidant and antibacterial activity against tested bacteria and there is the direct correlation between phyto chemistry and their antioxidant and antibacterial activity of plant.  

References:

1. Androw, J.M. 2001. BSAC Standardized disc susceptibility testing method. Journal Antimicrobial Chemotherapy, 7 (5): 48 - 57.


2. Bayramoglu, G., Bayramoglu, A., Engur, S., Senturk, H., Ozturk, N. and Colak, S. 2014. The hepatoprotective effects of Hypericum perforatum L. on hepatic ischemia/reperfusion injury in rats. Cytotechnology, 66(3): 443-448.


3. Burt, S. 2004. Essential oils: their antibacterial properties and potential application in foods-a review. International Journal of Food Microbiology, 94: 223-253.


4. Cai, Y., Luo, Q., Sun, M. and Corke, H. 2004. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer .Life Sciences, 74(17) :2157-2184.


5. Chang, C., Yang, M., Wen, H. and Chern, J. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal Food Drug Analaysis, 10(3): 178-182.


6. Cock, I.E. 2008. Antibacterial activity of selected Australian native plant extracts. The Internet Journal of Microbiology, 4(2): 1-8.


7. Eloff, J.N. 1998. Which extraction should be used for the screening and isolation of antimicrobial components from plants. Journal Ethnopharamacol, 60(1): 1-8.


8. Fabrican, D.S. and Farnsworth, N.R. 2001. The value of plants used in traditional medicine for drug discovery. Environ Health Perspect (EHP), 109(1): 69-75.


9. Fenner, R., Betti, A.H., Mentz, L.A. and Rates, S.M.K. 2006. Plants Used in  Brazilian folk medicine With potential antifungal activity. . Brazilian Journal of Pharmaceutical Sciences 2(3): 369-394.


10. Franklin, G., Conceica˜o, L.F.R., Kombrink, E. and Dias, A.C. 2009. Xanthone biosynthesis in Hypericum perforatum cells provides antioxidant and antimicrobial protection upon biotic stress. Phytochemistry, 70(1): 60–68.


11. Glisic, S., Popadic, S. and Skala, D. 2006. St. John’s Wort (Hypericum perforatum L.) –supercritical extraction, antimicrobial and antidepressant activity of extract and their components. Chem. Industry, 60: 61-72.


12. Khosravi, A. and Malecan, M. 2004. Effects of Lavandula stoechas extracts on staphylococcus aureus and other gram negative bacteria. The Journal of Qazvin University of Medical Sciences, 7(5): 3-9.


13. Kritika, N. 2007. Antimicrobial effect of five Zingiberaceae Essential oils. Molecules, 12: 2047-2060.


14. Mahboubi, M., Bokaee, S., Dehdashti, H. and Feizabadi, M.M. 2012. Antimicrobial activity of Mentha piperitae, Zhumeria majdae, Ziziphora tenuior oils on ESBLs producing isolates of Klebsiella pneumoniae, Biharean Biologist, 6(1): 5–9.


15. Mashreghi, M. and Momtazi, F. 2012. Comparison of the antibacterial effects of various concentrations of J. Rafsanjan Univ. Med. Sci. 11(2):103-14.


16. Kiaei E, Mazandarani M, Ghaemi A.The effect of ethanol extract of 7 species of  medicinal plants against bacteria isolated from patients with urinary tract infection in the city of Gorgan. J Med Plants. 2010; 9(2): 74-83.


17. Mazandarani, M., Makari, S. and Bajian, G.R. 2011. Evaluation of phytochemical and antioxidant activity in different parts of Heracleum gorganicum Rech.F. in Golestan province, North of Iran. Iranian J. plant physiology, 2(2): 381-388.


18. McDonald, S., Prenzler, P.D., Autolovich, M. and Robards, K. 2001. Phenolic content and antioxidant activity of olive extracts. Food Chemistry, 73:73-84.


19. Moore, L.B., Goodwin, B., Jones, S.A., Wisely, G.B., Serabjit-Singh, C.J.,  Willson, T.M., Collins, J.L. and Kliewer, S.  2000. Induces hepatic drug metabolism through activation of the pregnane X receptor. Proc Natl Acad Sci., 97(13): 7500–7502.


20. Mozdastan, S., Ebrahimzadeh, M.A. and Khalili, M. 2015. Comparing the impact of different extraction methods on antioxidant activities of myrtle (Myrtus communis L.). J. Mazandaran Univ Med Sci, 25(127): 10-24 (In Persian).


21. Mukherjee, P.K., Verpoorte, R. and Suresh, B. 2000. Evaluation of in vivo wound healing activity of Hypericum patulum (Family: Hypericaceae) leaf extract on different wound model in rats. J Ethnopharmacol, 70(3): 315–321.


22. Nostro, A., Germano, M.P., D’Aneglo, V., Marino, A. and Cannatelli, M.A. 2000. Extraction method and bioautography for evaluation of medicinal plant antimicrobial activity. Lett. Appl. Microbiol, 30(5): 379-384.


23. Galilee Marandi, R. 2012. Post harvest physiology. Third edition. Urmia: Urmia University Jihad publication, 624.


24. Radulovic, N., Stankov -Jovanovic, V., Stojanovic, G., Smelcerovic, A. Spiteller, M. and Asakawa, Y. 2007. Screening of in vitro antimicrobial and antioxidant activity of nine Hypericum species from the Balkans. Food Chemistry, 103(1): 15-21.


25. Sun, T., Powers, J.R. and Tang, J. 2007. Evaluation of the antioxidant activity of asparagus, broccoli and their juices. Food Chemistry, 105(1): 101-106.


26. Tortora, G.J., Funke, B.R. and Case, C.L. 2001. Microbiology: An Introduction, Benjamin Cummings, San Francisco. 12.


27. Trovato, A., Raneri, E., Kouladis, M., Tzakou, O., Taviano, M.F. and Galati, E.M. 2001. Anti inflammatory and analgesic activity of Hypericum empetrifolium Willd. (Guttiferae). Farmaco, 56: 455-457.


28. Wang, J., Kan, Q., Li, J., Zhang, X. and Qi, Y. 2011. Effect of neferine on liver ischemia–reperfusion injury in rats. Transplantation Proceeding, 43:2536–2539.


29. Weckesser, S., Engel, E., Simon, B., Wittmer, A., Pelz, K. and Schmepp, C.M. 2007. Screening of plant extracts for antimicrobial activity against bacteria and yeast with dermatological relevance. Phytomedicine, 14(7-8): 508-16.


30. WHO report, 2003. Global tuberculosis control: surveillance, planning, financing Geneva, Switzerland, (WHO/CDS/TB /2003.316).


31. Zarghami Moghaddam, P., Zolfaghari, M.R., Ghaemi, E.A., Mazandarani, M., Mansourian, A.R. and Taheri, S.A., 2011. Negative performance of root extract of Onosma dichroanthum Boiss. on the burn wound healing in an animal model. Archives of Clinical Microbiology. Microbial, 2(5): 1-5.