The study and comparison of phytochemical and antioxidant activity of various extracts of Dracocephalum moldavica L.in Sistan and Baluchestan province
Subject Areas : Phytochemistrymonireh mehrabi 1 , norolla hazeri 2 , Jafar Valizadeh 3 , Moharam Valizadeh 4
1 - Department of Chemistry, Faculty of Science, University of Sistan and Baluchestan, Zahedan, Iran
2 - Department of Chemistry, Faculty of Science, University of Sistan and Baluchestan, Zahedan, Iran
3 - 2Department of Biology, Faculty of Science, University of Sistan and Baluchestan, Zahedan, Iran
4 - 3 Department of Medicinal Plants, Faculty of Agriculture, University of Sistan and Baluchestan, Zahedan, Iran
Keywords: Anthocyanin, Flavonoid, Antioxidant, Dracocephalum moldavica L,
Abstract :
Dracocephalum moldavica L. is an annual herb belongs to Lamiaceae family, which has been used to treat of gastrointestinal, liver disorders and headaches.In this study, flowering branches of plant were collected from the greenhouse of the university of Sistan and Baluchestan in March 2016. The methanol, ethanol, and aqueous extracts of plant were obtained by using maceration method. The amount of total phenolic, flavonoid and anthocyanin contents were evaluated by spectrophotometric methods and the antioxidant activity of the extracts were determined by FRAP and DPPH methods. The results were showed that the phenolic and flavonoid contents different of methanol, ethanol and aqueous extracts were (82.10, 71.8and33.65 mg GAE/g) and (34.02, 28.8and1.02mg QUE/g), respectively. In the evaluation of antioxidant activity by DPPH, the inhibitory power of methanol, ethanol and aqueous extracts were (11.60, 34.93 and 298.96 μg/ml) respectively, and (14.23, 8.20 and 1.01 mM Fe2+/ mg) respectively, sample in FRAP assay. The methanol extract of plant had the greatest content of phenolic (82.10 mg GAE / g), flavonoids (34.02 mg QUE/g) and anthocyanin(113.55μg/g) and antioxidant activity compared to ethanol and aqueous extracts, especially it also had the greatest DPPH inhibitory power (IC50= 11.60 μg/ml).
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