Biochemical variation of phenolic and anthocyanin contents occurrence in pomegranate (Punica granatum L.) fruit juices among 25 genotypes of pomegranate cultivar "Malas"
Subject Areas : Chemistry and analysis of natural compoundsSeyed Abbas Mirjalili 1 , mehdi ghabooli 2 , elahe Poorazizi 3 , mitra aghajani 4
1 - plant production dept. imam khomeini higher education center, AREEO, tehran, iran
2 - faculty of agriculture and natural resources, Malayer university, malayer iran
3 - Biochemistry Deptpartment. Najafabad Branch, Islamic Azad University, Najafabad, Iran
4 - faculty of agriculture and natural resources, malayer university, malayer iran
Keywords: Antioxidant, Anthocyanin, Polyphenol, pomegranate cultivar Malas, soluble solids,
Abstract :
Pomegranate (Punica granatum L.) is a native plant of Iran which cultivated throughout the country. Various metabolites and bioactive compounds have been reported from it. The plant has highly antioxidant potentials and possesses considerable phenolic ingredients. In order to study physico-chemical properties and qualitative attributes of pomegranate fruit juices in 25 genotypes of cultivar Malas, as the most famous Iranian cultivar, the experiment was accomplished in Agricultural Biotechnology Research Institute in Isfahan at October 2015. The fruits harvested from Pomegranate Genotypes Genetic Resources of Iran in yazd. Anthocyanin, polyphenol contents (by Folin-Ciocalteu), antioxidant capacity (by DPPH), total soluble solids and total acidity as the biochemical traits and taste, aril color, peel color, quality of aril color and aril quality as the qualitative characteristics were measured. Results showed that the highest acidity found in Malas Zoodras Kan and total soluble solids in Malas Naalout Baneh (18.53 Brix). Malas Nar Poost Ghermez Marivan had maximum antioxidant capacity. Malas Daneh Siah Bafgh and Malas Larz Galoubarik Ardestan had the highest contents of anthocyanin and polyphenols, respectively. Study of simple correlation among the attributes had no significant differences. According to the same conditions subjected to all plants in the collection, it concluded the premier genotypes could be used as applied purposes such as pomegranate anthocyanin production and/or high antioxidant.
References
Adhami, V.M. andMukhtar, H.2006.Polyphenols from green tea and pomegranate for prevention of prostate cancer. Free radical research, 40(10):1095-1104.
Alighourchi, H., Barzegar, M. and Soleiman, A. 2007.Anthocyanins characterization of 15 Iranian pomegranate (PunicagranatumL.) varieties and their variation after cold storage and pasteurization. European Food Research and Technology, 227(3): 881-887.
Apak, R., Güçlü, K., Demirata, B., Özyürek, M., Çelik, S.E., Bektaşoğlu, B., Berker, K. I., and Özyurt, D. 2007. Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the cupric assay. Molecules, 12: 1496–1547.
Barzegar, M., Fadavi, A. and Azizi, T.M.H. 2004.Evaluation of physico-chemical composition of cultivated pomegranates in Yazd. Iranian Journal of Nutrition Science and Food Technology 1 (2): 9-14. (in Persian)
Borochov-Neori, H., Judeinstein, S., Tripler, E., Harari, M., Greenberg, A., Shomer, I., and Holland, D. 2009.Seasonal and cultivar variations in antioxidant and sensory quality of pomegranate (PunicagranatumL.) fruit. Journal of Food Composition and Analysis, 22: 189-195.
Fadavi, A., Barzegar, M., Azizi, M.H., andBayat, M. 2005. Physicochemical composition of ten pomegranate cultivars (Punicagranatum L.) grown in Iran. Food Science and Technology International, 11(2): 113-119.
Feyzi, F., Seifi E., Varasteh, F., Hemmati, Kh.,Fereydooni, H. 2015.evalution of some biochemical properties of two pomegranate cultivars in three different region. 4th national congress on medicinal plants, Tehran, Iran.
Gil, M.I., Tomas-Barberan, F.A., Hess-Pierce, B., Holcroft, D.M., and Kader, A.A. 2000.Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing.Journal of Agricultural and Food Chemistry 48: 4581- 4589.
Giusti, M.M., Wrolstad, R.E. 2001.Characterization and measurement of anthocyanins by UV–visible spectroscopy. In: Wrolstad, R.E., Schwartz, S.J. (Eds.),Current Protocols in Food Analytical Chemistry. John Wiley and Sons, New York.
Jaiswal, V., Der Marderosian, A. and Porter, J.R. 2009.Anthocyanins and polyphenol oxidase from dried arils of pomegranate (PunicagranatumL.). Food Chemistry, 118: 11-16.
JaliliMoghadam Z. 2015.A comprehensive guide for pomegranate cultivation.Agricultural Education and extension publishing.Tehran (in Persian).348 p.
Lansky, E.P. & Newman, R.A. 2007.Punicagranatum(pomegranate) and its Potential Forprevention and treatment of inflammation and Cancer. Journal of Ethnobotany and Pharmacology, 109: 177-2060.
Madrigal Carballob, S., Rodriguezb, G., Kruegera, C.G., Dreherc, M., and Reeda, J.D. 2009. Pomegranate (Punicagranatum) supplements: Authenticity, antioxidant and polyphenol composition. Journal of Functional Foods, 1: 324-329.
Melgarejo, P., Salazar, D.M., andArtes, F. 2000.Organic acids and sugars composition of harvested pomegranate fruits. European Food Research and Technology, 211(3): 185-190.
Miguel, G., Fontes, C., Antunes, D., Neves, A. and Martins, D. 2004. Anthocyanin concentration of “Assaria” pomegranate fruits during different cold storage conditions. BioMed Research International, 5: 338-342.
Mirdehghan, S.H., andRahemi, M. 2007. Seasonal changes of mineral nutrients andphenolics in pomegranate (PunicagranatumL.) fruit. Journal of ScientiaHorticulturae, 111: 120–127.
Mirjalili, S.A. 2015.A review on Biochemical constituents and medicinal properties of pomegranate (PunicagranatumL). Journal of Medicinal Plants, 4(56):1-22 (in Persian).
Mirjalili, S.A. 2016.Pomegranate, biodiversity and genetic resources.Rostaniha, 17(1): 1-18. (In Persian)
Mousavinejad, G., Emam-Djomeh, Z., Rezaei, K., andKhodaparast, M.H.H. 2009.Identification and quantification of phenolic compounds and their effects on antioxidant activity in pomegranate juices of eight Iranian cultivars. Food Chemistry, 115: 1274-79.
Pokorny, J., Yanishlieva, N., and Gordon, M. 2001.Antioxidants in Food.Practical pomegranates grown in Turkey. Journal of Food Composition and Analysis, 15: 567-575.
Seeram, N.P., Aviram, M., Zhang, Y., Henning, S.M.,Feng, L., Dreher, M., Heber, D. 2008.Comparison of antioxidant potency of commonly consumed polyphenolrich beverages in the United States. Journal of Agriculturaland Food Chemistry, 56: 1415–1422.
Shahidi, F., and Naczk, M. 2004.Phenolic in food and nutraceuticals. Pp. 131–239.
Sun, T. and Ho, C.T. 2005. Antioxidant activity of buck wheat extracts. FoodChemistry, 90: 743 – 749.
Tatari, M., Ghazvini, R. F., Ghasemnejad, M., Mousavi, S.A., and Tabatabaii, S.Z. 2011.Morphological and biochemical characteristics of fruit in some pomegranate cultivars in climatical conditions of Saveh. Seed and Plant Improvement Journal, 27(1): 69-87. (in Persian)
Tehranifar, A., Zarei, M., Nemati, Z., Esfandiyari, B., Vazifeshenas, M.R. 2010.Investigation of physico-chemical properties and antioxidant activity of twenty Iranian pomegranate (PunicagranatumL.) cultivars.ScientiaHorticulturae, 126(2):180-185.
Tezcan, F., Gultekin-Ozguven, M., Diken, T., Ozcelik, B., Erim, F.B. 2009. Antioxidant activity and total phenolic, organic acid and sugar content in commercial pomegranate juices. Food Chemistry, 115: 873–877.
Tzulker, R., Glazer, I., Bar-Ilan, I., Holland, D., Aviram, M., and Amir, R. 2007. Antioxidant activity, polyphenol content, and related compounds in different fruit juices and homogenates prepared from 29 different pomegranate accessions. Journal of Agricultural and Food Chemistry, 55: 9559–9570.
Varidi, M.J. 1992. Chemical compositions and clarification probability of pomegranate extract. M. Sc. Thesis of Food Industry, TarbiatModarres University.
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