The effect of foliar application of chitosan and shrimp skin powder on photosynthetic pigments, some growth parameters and essential oil of the medicinal plant Hyssopus officinalis
Fatemeh Khosheghbal ghorabaee
1
(
Dept. of Biology, Faculty of Science, Payame Noor University,, Tehran,, Iran
)
َAbdollah Ghasemi Pirbalouti
2
(
Research Center for Medicinal Plants, Shahr-e- Qods Branch, Islamic Azad University, Tehran, Iran
)
Shekoofeh Enteshari
3
(
Dearptment of Biology, Faculty of Science, Payame Noor University, Tehran, Iran
)
seyed Javad Davarpanah
4
(
Applied Biotechnology Research Center, Baqiyatullah University of Medical Sciences, Tehran, Iran
)
Keywords: : ʺchitosanʺ, ʺessential oilʺ , ʺHyssopʺ , ʺshrimp skinʺ,
Abstract :
One of the methods to increase the secondary metabolites production in plants is to use elicitors and chitosan is a biotic elicitor obtained from the deacetylation of chitin in the outer shell of crustaceans such as shrimp. Shrimp skin is part of shrimp solid waste discarded in large amount every year. This research was carried out to investigate the effect of foliar application of chitosan elicitor (0.25 and 0.5 g/l) and shrimp skin powder (2.5 and 5 g/L), on medicinal plant Hyssopus officinalis in potted form at the Experimental Farm of Islamic Azad University, Shahre-kord Branch during spring to fall 2017. The essential oils obtained from aerial part of hyssop by hydro-distillation. The essential oils were analyzed by GC-FID and GC/MS. Totally, 33 compounds identified representing more than 89-95% of the oil composition. The predominant components of the essential oil in all treatments were cis-pinocamphon (43.19-55.09%) and beta-pinene (8.31-15.04%). Foliar application of chitosan, shrimp skin and shrimp skin × chitosan increased the percentage of essential oil and cis-pinocamphon, but statistically only the effect of chitosan 0.5 was significant. Chitosan treatments decreased plant height, shoot fresh and dry weight, chlorophyll a, chlorophyll b and total chlorophyll of hyssop but shrimp skin and chitosan × shrimp skin solutions increased. Although, no one was significant. According to the results of this study, it seems that in addition to chitosan, shrimp skin with higher concentrations can be used to increase effective substances of the medicinal plant Hyssopus officinalis.
