Histopathological evidences for effect beneficial of Satureja hortensis extract on hepatic lesion by cadmium–induced in Rat
محورهای موضوعی : مجله گیاهان دارویی
الهام مقتدایی خوراسگانی
1
,
عبداله قاسمی پیربلوطی
2
,
ش. ادیبی
3
1 - گروه پاتولوژی، دانشکده دامپزشکی، دانشگاه ازاد واحد شهرکرد، شهرکرد، ایران
2 - مرکز پژوهش های گیاهان دارویی و دام پزشکی سنتی، دانشگاه آزاد اسلامی واحد شهرکرد، شهرکرد، ایران؛
بخش تحقیقات گیاهان دارویی، دانشکده کشاورزی استاکبریج، کالج علوم طبیعی، دانشگاه ایالتی ماساچوست، آمریکا
3 - مرکز تحقیقات دامپزشکی ترابی نژاد، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
کلید واژه: Cadmium, Medicinal herbs, aspartate transminase (AST), alanine transaminase (ALT), Liver histopathology,
چکیده مقاله :
Background & Aim:Cadmium is an important industrial and environmental pollutant. Cadmium is one of the most toxic and carcinogenic heavy metals to organisms. This heavy metal mainly distributes to the liver and kidney in humans and animal and, causing acute hepatic injury. Experimental: The ethanol extract of Satureja hortensis L. (Lamiaceae family), was evaluated for its activity against cadmium–induced in male Wister rats (150 – 180 g). The ethanol extract of S. hortensis (100 and 200 mg/kg/day for six weeks) was examined on serum bicochemical and hepatic histopathological characteristic of rats subcutaneously received with cadmium chloride (CdCl2) at 3 mg/kg/day for six weeks. Results: The biochemical results indicated that aspartate transminase (AST) and alanine transaminase (ALT) significantly increased in serum by cadmium–induced. The liver histopathological results revealed that the ethanol extract of S. hortensis treatment at 200 mg/kg/day significantly reduced toxicity by cadmium–induced. The ethanol extract of S. hortensis prevents the cadmium–induced lesions in hepatic function. Recommended applications/industries: Known antioxidant, antimicrobial, antihepatotoxic, nephroprotective potentials of the extract of S. hortensis may be the mechanisms by which this plant protects animals against experimentally cadmium–induced.
مقدمه و هدف: کادمیوم یک آلاینده مهم صنعتی و زیست محیطی می باشد و یکی از سمی ترین فلزات سنگین سرطانزابرای ارگانیسمها قلمداد می شود. این فلز سنگین عمدتا در کبد و کلیه انسان و حیوانات تجمع یافته و باعث آسیب کبدی می شود. روش تحقیق: عصاره تانولی Satureja hortensis L برای ارزیابی فعالیت آن در موشهای نر(180-150 گرم) هپاتیتی شده با کادمیوم مورد بررسی قرار گرفت و تاثیر مقدار 100 و 200 میلی گرم /کیلوگرم / روز و به مدت 6 هفته بر ویژگیهای بیوشیمیایی سرم و هیستوپاتولوژی کبد در موشهایی که کلرید کادمیوم به میزان 3 میلی گرم/ کیلوگرم/ روز در طول این مدت دریافت کرده بودند ارزیابی شد. نتایج و بحث: نتایج آزمایشات بیوشیمیایی نشان داد که آسپارتات ترانس آمیناز و آلانین ترانس آمیناز به طور معنی دار در سرم افزایش یافت. نتایج هیستوپاتولوژی کبد مشخص کرد که عصاره اتانولی S. hortensis در مقدار 200 میلی گرم/کیلوگرم/روز به طور قابل توجه سمیت القا شده توسط کادمیوم را کاهش داد و این عصاره از آسیب کبدی ناشی از کادمیوم ممانعت به عمل آورد. توصیه های کاربردی/صنعتی: پتانسیل آنتی اکسیدانی، ضد میکروبی، ممانعت کنندگی از مسمومیت کبدی و آسیب کلیوی در عصاره S. hortensis از عواملی هستند که از طریق آن این گیاه از حیوانات در برابر آسیبهای القا شده توسط کادمیوم محافظت می کند.
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