Recognition of Sulfur Compounds in Tissue Culture Different Organs of Persian Shallot (Allium hirtifolium Boiss) by GC/MS

محورهای موضوعی : مجله گیاهان دارویی

مهتاب اصفهانی زاده حسین پور ¹ (باشگاه پژوهشگران جوان و نخبگان، واحد اصفهان(خوراسگان)، دانشگاه آزاد اسلامی، اصفهان، ایران)
فروغ مرتضایی نژاد ² (گروه علوم باغبانی، واحد اصفهان(خوراسگان)، دانشگاه آزاد اسلامی، اصفهان، ایران)

تاریخ دریافت : 1393/11/05 تاریخ پذیرش : 1393/12/25 تاریخ انتشار : 1394/10/11

کلید واژه: In vitro culture, Medicinal plant, Headspace, Stem-disc bulb,

چکیده مقاله :

Background & Aim: Persian shallot (Allium hirtifolium Boiss) with common name of "Musir" is one of the endemic medicinal plants in Iran. Leaves and bulbs with antibacterial, antifungal, anticancer, anti-diabetes and anti-obesity effects are edible parts of this plant. The tissue culture is an influential method to multiply plant productions in order to meet demands of pharmacy and food industries. Therefore this article will focus on levels and structures of sulfur compounds in seeds, grassland bulbs and compare them with those of leaves, roots, bulbs and callus obtained from the tissue culture using the headspace- Gas chromatography-Mass Spectrophotometry (GC/MS) method. Experimental: At the first of the study, seeds and stem-discs of grassland bulbs were cultured in MS media. After 90 days seeds, grassland bulbs, seed-leaves, seed-bulbs, leaves and roots of seed-bulbs, stem-disc bulbs, and callus of stem-discs analyzed by GC/MS-headspace method. Results & Discussion: The results of analysis showed the highest variety of sulfur compounds was in seed bulb roots, while lowest levels were found in seeds, seed bulb leaves and callus. And also the highest level of sulfur compounds was discovered in stem-disc bulbs (28.31%), seed bulb roots (18.61%) and grassland bulbs (6.23%). Industrial and practical recommendations: Our findings has been shown Persian shallot sulfur compounds have mono, di and tri sulfur in structures and the best source for these metabolites were roots and stem-disc bulbs producing by in vitro culture method.

چکیده انگلیسی:

مقدمه و هدف:  موسیر ایرانی (Allium hirtifolium Boiss)با نام عمومی موسیر، یکی از گیاهان دارویی بومی ایران می‌باشد. قسمت­های خوراکی این گیاه شامل برگ و پیازها هستندکه دارای  اثرات ضدباکتری، ضدقارچ، ضدسرطان، ضد دیابت و ضدچاقی می­باشند.  با توجه به این که تکنیک کشت بافت توانایی تولید چند برابر ظرفیت از گیاهان را جهت پاسخگویی به نیاز صنعت غذا و دارو دارد. بنابراین، این مقاله  بر بررسی سطح و ساختار ترکیبات سولفیددار موجود در بذر و پیاز حاصل از مرتع و مقایسه آن­ها با برگ، ریشه ، پیاز و کالوس­های به دست آمده از طریق کشت بافت توسط دستگاه کروماتوگرافی گازی متصل به طیف سنج جرمی، متمرکز می­باشد. روش تحقیق: در این بررسی ابتدا بذر و طبق پیاز مرتع در محیط کشت MS کشت گردید. بعد از گذشت ٩٠ روز بذر، پیاز مرتع،  برگ بذر، پیاز بذر، برگ و ریشه­های پیاز بذری، پیاز حاصل از طبق و کالوس حاصل از کشت طبق با روش Head space گاز کروماتوگرافی متصل به طیف سنج جرمی بررسی شدند. نتایج و بحث: آنالیز نتایج نشان داد بیشترین گوناگونی ترکیبات سولفور دار در ریشه پیاز بذری و کمترین سطح در بذرها، برگهای پیاز بذری و کالوس مشاهده گردید. همچنین بیشترین سطح ترکیبات سولفوردار در پیاز طبق(28.31%)، ریشه پیاز بذری(18.61%) و پیاز مرتع(6.23 %) مشاهده گردید. توصیه کاربردی/ صنعتی: یافته­های ما با روش Head space گاز کروماتوگرافی متصل به طیف سنج جرمی نشان دادترکیبات سولفوردار موسیر ایرانی شامل ساختارهای مونو، دی و تری سولفور می­باشد و بهترین منبع برای این متابولیت­ها ریشه ها و پیاز حاصل از طبق است که به روش درون شیشه ای تولید گردد.

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