Comparison of Fresh and Cryopreserved Semen Quality of Polled and Horned Bali Bulls

محورهای موضوعی : Camel

H. Hasbi ¹ (Faculty of Animal Science, Hasanuddin University, South Sulawesi, Indonesia)
M.I.A. Dagong ² (Faculty of Animal Science, Hasanuddin University, South Sulawesi, Indonesia)
Z. Zulkharnain ³ (Faculty of Animal Science, Hasanuddin University, South Sulawesi, Indonesia)
S. Baba ⁴ (Faculty of Animal Science, Hasanuddin University, South Sulawesi, Indonesia)
H. Sonjaya ⁵ (Faculty of Animal Science, Hasanuddin University, South Sulawesi, Indonesia)
S. Baco ⁶ (Faculty of Animal Science, Hasanuddin University, South Sulawesi, Indonesia)
S. Gustina ⁷ (Department of Animal Science, Faculty of Animal Science and Fisheries, Universitas Sulawesi Barat, Majene, Indonesia)
T. Maulana ⁸ (Research Center for Applied Zoology, Research Organization for Life Science, National Research and Innovation Agency (BRIN), West Java, Indonesia)
M. Gunawan ⁹ (Research Center for Applied Zoology, Research Organization for Life Science, National Research and Innovation Agency (BRIN), West Java, Indonesia)
P.P. Agung ¹⁰ (Research Center for Applied Zoology, Research Organization for Life Science, National Research and Innovation Agency (BRIN), West Java, Indonesia)
N. Herlina ¹¹ (Research Center for Applied Zoology, Research Organization for Life Science, National Research and Innovation Agency (BRIN), West Java, Indonesia)
N.D. Yanthi ¹² (Research Center for Applied Zoology, Research Organization for Life Science, National Research and Innovation Agency (BRIN), West Java, Indonesia)
E.M. Kaiin ¹³ (Research Center for Applied Zoology, Research Organization for Life Science, National Research and Innovation Agency (BRIN), West Java, Indonesia)
S. Said ¹⁴ (Research Center for Applied Zoology, Research Organization for Life Science, National Research and Innovation Agency (BRIN), West Java, Indonesia)

تاریخ دریافت : 1401/04/07 تاریخ پذیرش : 1401/07/30 تاریخ انتشار : 1401/12/10

کلید واژه: breed, Bali bulls, horned, polled, sperm characteristics,

چکیده مقاله :

A purpose of study is to examine sperm quality of Bali polled and horned bulls. The semen samples collected from 4 Bali polled bulls and 4 horned bulls aged between 5 and 7 years using an artificial vagina, then evaluated macroscopically and microscopically. A macroscopic evaluation revealed no significant differences of semen between polled and horned cattle. However, the microscopic evaluation revealed that sperm concentration, motility, and abnormality of polled bulls were significantly lower than the horned bulls, and there was no apparent difference between viability and membrane integrity. Except for abnormalities and DNA integrity of spermatozoa, all parameters of sperm quality were not significantly different between polled and horned bulls. Sperm abnormalities in polled bulls were significantly higher than in horned ones. However, sperm DNA integrity of the polled bulls was significantly higher than horned bulls. The analysis of CASA revealed that most kinematics were higher in polled bull spermatozoa than in horned ones, except for wobble (WOB) or ration of velocity average path (VAP) and velocity curvilinear (VCL) and amplitude of lateral head displacement (ALH), which were higher in horned bulls than in polled bulls. We therefore concluded that the quality of fresh sperm from horned bulls was better than polled bulls. However, polled bulls semen has greater freezing ability than the horned ones. Furthermore, the polled and horned bulls had the same semen characteristics that enable them for fertilizing ability, and both bulls have good DNA integrity.

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